Tong Lei, Liu Jin-Li, Wang Jing-Xin, Sun Lin-Lin, Song Yi-Ling, Tian Gui-Yuan, He Shuai, Gao Yue-Juan
School of Pharmacy, Mudanjiang Medical University, Mudanjiang 157011, China.
Hongqi Hospital, Mudanjiang Medical University, Mudanjiang 157011, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):728-730. doi: 10.4268/cjcmm20160431.
To investigate the effects of Yupingfeng granule (YPF) on immune factors of the rats with allergic rhinitis (AR) induced by ovalbumin(OVA). OVA 0.3 mg, Al(OH)3 30 mg and saline 1 mL were mixed and intraperitoneally injected for the initial immunization, 4% OVA 200 μg (50 μL) was given to the nose on the 15th day for the second immunization to establish the allergic rhinitis model. Sixty male SD rats were randomly divided into allergic rhinitis(AR) model group, Yupingfeng granule three dose (2.7,1.35,0.68 g•kg⁻¹) groups, control drug Biyankang (0.4 g•kg⁻¹) and normal control group. After 14 days, efforts were made to collect blood from abdominal aorta, and take nasopharynx tissues and fasten them into 10% formaldehyde for a pathological examination. The levels of HIS, IgE, IL-4 and TNF-α in serum were examined by radioimmunoassay, and nasal mucosa tissues were examined by HE staining. According to the results, the levels of HIS, IgE, IL-4 and TNF-α in serum of Yupingfeng granule groups were significantly lower than that of AR model group (P<0.05, P<0.01). Nasal mucosa tissues showed slight morphological changes and inflammatory cell infiltration, with unobvious necrosis. Yupingfeng granule can improve the pathological changes of nasal mucosa tissues, and reduce the production and release of immune factors during allergic rhinitis (AR) process in vivo by OVA, which may be the important curative mechanism of allergic rhinitis.
探讨玉屏风颗粒（YPF）对卵清蛋白（OVA）诱导的变应性鼻炎（AR）大鼠免疫因子的影响。将0.3 mg OVA、30 mg氢氧化铝和1 mL生理盐水混合后腹腔注射进行初次免疫，于第15天给予4% OVA 200 μg（50 μL）滴鼻进行二次免疫以建立变应性鼻炎模型。将60只雄性SD大鼠随机分为变应性鼻炎（AR）模型组、玉屏风颗粒三个剂量（2.7、1.35、0.68 g•kg⁻¹）组、对照药物鼻炎康（0.4 g•kg⁻¹）组和正常对照组。14天后，经腹主动脉采血，取鼻咽组织并固定于10%甲醛中进行病理检查。采用放射免疫法检测血清中组胺（HIS）、免疫球蛋白E（IgE）、白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）水平，鼻黏膜组织行苏木精-伊红（HE）染色检查。结果显示，玉屏风颗粒各剂量组血清中HIS、IgE、IL-4和TNF-α水平均显著低于AR模型组（P<0.05，P<0.01）。鼻黏膜组织形态学改变轻微，有炎性细胞浸润，未见明显坏死。玉屏风颗粒可改善鼻黏膜组织的病理变化，减少OVA在体内诱导的变应性鼻炎（AR）过程中免疫因子的产生和释放，这可能是其治疗变应性鼻炎的重要作用机制。
