Ghosal Sandip
Department of Mechanical Engineering and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, IL, USA.
Methods Mol Biol. 2019;1906:143-166. doi: 10.1007/978-1-4939-8964-5_11.
In capillary electrophoresis (CE), analytes are separated along the axis of a single microcapillary by virtue of their differential migration in an applied electric field. CE can also be performed in channels etched on solid substrates such as glass or PDMS and can be integrated into a microfluidic chip with a complex network of electric and fluidic circuits. The measure of quality of a CE instrument is resolution which is limited fundamentally by mixing due to various physical processes. The theoretical limit on the best separation that can be achieved is set by molecular diffusion, which is inevitable. The goal is to eliminate or minimize the other sources of dispersion by design. This chapter provides an overview of the various mechanisms of band broadening and the mathematical results that make it possible to estimate their relative contributions.
在毛细管电泳（CE）中，分析物凭借其在施加电场中的不同迁移率，沿单个微毛细管的轴线进行分离。CE 也可在蚀刻于诸如玻璃或聚二甲基硅氧烷等固体基质上的通道中进行，并且可以集成到具有复杂电路和流体电路网络的微流控芯片中。CE 仪器质量的衡量标准是分辨率，而分辨率从根本上受到各种物理过程导致的混合的限制。能够实现的最佳分离的理论极限由分子扩散设定，这是不可避免的。目标是通过设计消除或最小化其他分散源。本章概述了谱带展宽的各种机制以及能够估计它们相对贡献的数学结果。
