Hong S, Lee C S
Department of Chemistry and Ames Laboratory, Iowa State University 50011, USA.
Electrophoresis. 1995 Nov;16(11):2132-6. doi: 10.1002/elps.11501601344.
The resolution in capillary zone electrophoresis (CZE), with the assumption of diffusion control only, is strongly dependent on the direction and magnitude of electroosmotic flow. In fact, excellent separation resolution will be obtained if the electroosmotic flow is in the opposite direction of the electrophoretic migration. By applying various radial electric potential gradients across the capillary wall, the direct control of the zeta potential and the electroosmotic flow results in a great enhancement of chiral resolution in cyclodextrin-modified CZE. All 12 basic chiral drugs, including (+/-)-ephedrine, (+/-)-pseudoephedrine, (+/-)-norephedrine, (+/-)-epinephrine, (+/-)-norepinephrine, and (+/-)-isoproterenol, are separated and resolved within 22 min of the analysis time. There is no additional band broadening and dispersion introduced by the direct control of electroosmosis with the application of various radial electric potential gradients.
在仅假设受扩散控制的毛细管区带电泳（CZE）中，分离度强烈依赖于电渗流的方向和大小。事实上，如果电渗流与电泳迁移方向相反，将获得出色的分离度。通过在毛细管壁上施加各种径向电势梯度，直接控制zeta电位和电渗流可极大提高环糊精修饰的CZE中的手性分离度。所有12种碱性手性药物，包括（+/-）-麻黄碱、（+/-）-伪麻黄碱、（+/-）-去甲麻黄碱、（+/-）-肾上腺素、（+/-）-去甲肾上腺素和（+/-）-异丙肾上腺素，在22分钟的分析时间内实现分离和拆分。通过施加各种径向电势梯度直接控制电渗作用，不会引入额外的谱带展宽和分散。
