Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People's Republic of China.
Pest Manag Sci. 2019 Jun;75(6):1646-1654. doi: 10.1002/ps.5282. Epub 2019 Jan 7.
Sulfoxaflor is a new insecticide for controlling Nilaparvata lugens in the field. This study was conducted to investigate the risk of resistance development, the cross-resistance spectrum and the mechanisms of sulfoxaflor resistance in N. lugens.
A sulfoxaflor-resistant strain was obtained from a field population by successive selection with sulfoxaflor for 39 generations in the laboratory. Sulfoxaflor-resistant populations showed significant levels of cross-resistance to dinotefuran, nitenpyram, thiamethoxam, clothianidin, imidacloprid and cycloxaprid. However, they exhibited only minor or no cross-resistance to isoprocarb, etofenprox, chlorpyrifos, triflumezopyrim and buprofezin. Sulfoxaflor was synergized by the inhibitor piperonyl butoxide (PBO) in the sulfoxaflor-resistant strain (SFX-SEL) with 2.69-fold relative synergistic ratios compared with the unselected strain (UNSEL). Compared with UNSEL, the P450 enzyme activity of SFX-SEL was increased 3.50 times, and eight P450 genes were upregulated more than 2.0-fold in SFX-SEL. RNAi reduced the expression of CYP6ER1 (36.87-fold change) and significantly enhanced the susceptibility of SFX-SEL to sulfoxaflor.
Resistance development and cross-resistance risk of sulfoxaflor-resistance in N. lugens is evident. The enhanced detoxification of P450 enzymes caused by upregulation of several P450 genes is considered to be the metabolic resistance mechanism. These results suggest that CYP6ER1 might play an important role in sulfoxaflor resistance in N. lugens. © 2018 Society of Chemical Industry.
噻虫砜是一种新型杀虫剂，可用于田间防治褐飞虱。本研究旨在调查褐飞虱对噻虫砜产生抗药性的风险、抗药性的交互抗性谱和机制。
通过在实验室中连续 39 代用噻虫砜对田间种群进行选择，获得了对噻虫砜具有抗性的种群。噻虫砜抗性种群对啶虫脒、吡虫啉、噻虫嗪、噻虫啉、氯噻啉和噻虫氟氯氰具有显著的水平的交互抗性。然而，它们对异丙威、乙虫腈、毒死蜱、三氟氯氰菊酯和噻嗪酮仅表现出轻微或无交叉抗性。噻虫砜在噻虫砜抗性种群（SFX-SEL）中与抑制剂增效醚（PBO）协同作用，与未选择的种群（UNSEL）相比，相对增效比为 2.69 倍。与 UNSEL 相比，SFX-SEL 的 P450 酶活性增加了 3.50 倍，SFX-SEL 中有 8 个 P450 基因的表达上调了 2.0 倍以上。RNAi 降低了 CYP6ER1 的表达（变化倍数为 36.87 倍），并显著增强了 SFX-SEL 对噻虫砜的敏感性。
褐飞虱对噻虫砜的抗药性发展和抗药性风险明显。几种 P450 基因的上调导致 P450 酶的解毒作用增强，被认为是代谢抗性机制。这些结果表明，CYP6ER1 可能在褐飞虱对噻虫砜的抗性中发挥重要作用。© 2018 化学工业协会。
