Institute of Crop Protection, Guizhou University, Guiyang, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
Insect Sci. 2021 Feb;28(1):115-126. doi: 10.1111/1744-7917.12764. Epub 2020 Apr 13.
The brown planthopper is a notorious rice pest in many areas of Asia. The evolution of insecticide resistance in Nilaparvata lugens has become a serious problem in the effective control of this pest in the paddy field. In this article, the current susceptibility of N. lugens field populations to novel mesoionic insecticide triflumezopyrim and major classes of chemical insecticides was determined and compared. The monitoring results indicated that field populations of N. lugens had developed low resistance to triflumezopyrim (resistance ratio, RR: 1.3-7.3-fold) during 2015-2018 in China, and the median lethal concentration values varied from 0.05 to 0.29 mg/L. Additionally, during 2017 to 2018, field populations of N. lugens showed high resistance levels to thiamethoxam (RR: 456.1-1025.6-fold), imidacloprid (RR: 2195.3-6899.0-fold) and buprofezin (RR: 1241.5-4521.7-fold), moderate to high resistance levels to dinotefuran (RR: 97.6-320.1-fold), clothianidin (RR: 69.4-230.1-fold) and isoprocarb (RR: 44.1-108.0-fold), and low to moderate levels of resistance to chlorpyrifos (RR: 12.0-29.7-fold) and nitenpyram (RR: 6.9-24.1-fold). In contrast, N. lugens just showed low resistance to sulfoxaflor (RR: 3.3-8.5-fold) and etofenprox (RR: 5.0-9.1-fold) in the field. Additionally, the P450 gene CYP6ER1 was found to be significantly overexpressed in all five field populations of N. lugens collected in 2018 when compared with a laboratory susceptible strain. Our findings will provide useful information to delay the evolution of insecticide resistance in N. lugens.
褐飞虱是亚洲许多地区一种声名狼藉的水稻害虫。在稻田中有效控制这种害虫的过程中，褐飞虱对杀虫剂的抗药性进化已成为一个严重的问题。在本文中，测定并比较了新型亚氨基类杀虫剂三氟苯嘧啶和主要类别的化学杀虫剂对褐飞虱田间种群的当前敏感性。监测结果表明，2015-2018 年中国褐飞虱田间种群对三氟苯嘧啶的抗药性发展缓慢（抗性比 RR：1.3-7.3 倍），LC50 值在 0.05-0.29mg/L 之间。此外，2017-2018 年，褐飞虱田间种群对噻虫嗪（RR：456.1-1025.6 倍）、吡虫啉（RR：2195.3-6899.0 倍）和噻嗪酮（RR：1241.5-4521.7 倍）表现出高抗水平，对啶虫脒（RR：97.6-320.1 倍）、氯虫苯甲酰胺（RR：69.4-230.1 倍）和异丙威（RR：44.1-108.0 倍）表现出中高抗水平，对毒死蜱（RR：12.0-29.7 倍）和吡虫隆（RR：6.9-24.1 倍）表现出低至中抗水平，而对硫氟肟醚（RR：3.3-8.5 倍）和乙虫腈（RR：5.0-9.1 倍）仅表现出低抗水平。此外，与实验室敏感品系相比，2018 年采集的 5 个褐飞虱田间种群的 P450 基因 CYP6ER1 显著过表达。我们的研究结果将为延迟褐飞虱对杀虫剂的抗药性进化提供有用的信息。
