Institute of Crop Protection, Guizhou University, Guiyang 550025, PR China; The Provincial Key Laboratory for Agricultural Pest Management in Mountainous Region, Guizhou University, Guiyang 550025, PR China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Pestic Biochem Physiol. 2023 Aug;194:105467. doi: 10.1016/j.pestbp.2023.105467. Epub 2023 May 16.
Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR), a crucial electron-transfer partner of P450 systems, is required for various biological reactions catalyzed by P450 monooxygenase. Our previous study indicated that enhanced P450 enzyme detoxification and CYP6ER1 overexpression contributed to sulfoxaflor resistance in Nilaparvata lugens. However, the association between CPR, sulfoxaflor resistance, and neonicotinoid cross-resistance in N. lugens remains unclear. In this study, the sulfoxaflor-resistant (SFX-SEL) (RR = 254.04-fold), resistance-decline (DESEL) (RR = 18.99-fold), and susceptible unselected (UNSEL) strains of N. lugens with the same genetic background were established. Real-time quantitative polymerase chain reaction (RT-qPCR) revealed that the N. lugens CPR (NlCPR) expression level in the SFX-SEL strain was 6.85-fold and 6.07-fold higher than in UNSEL and DESEL strains, respectively. NlCPR expression was significantly higher in the abdomens of UNSEL, DESEL, and SFX-SEL fourth-instar nymphs than in other tissues (thoraxes, heads, and legs). Additionally, sulfoxaflor stress significantly increased NlCPR mRNA levels in the UNSEL, SFX-SEL and DESEL strains. NlCPR silencing by RNA interference (RNAi) dramatically increased the susceptibility of the UNSEL, DESEL, and SFX-SEL strains to sulfoxaflor, but the recovery of SFX-SEL was more obvious. Furthermore, NlCPR silencing led to a significant recovery in susceptibility to nitenpyram, dinotefuran, clothianidin, and thiamethoxam across all strains (UNSEL, DESEL, and SFX-SEL), with the greatest degree of recovery in the sulfoxaflor-resistant strain (SFX-SEL). Our findings suggest that NlCPR overexpression contributes to sulfoxaflor resistance and neonicotinoid cross-resistance in N. lugens. This will aid in elucidating the significance of CPR in the evolution of P450-mediated metabolic resistance in N. lugens.
烟酰胺腺嘌呤二核苷酸磷酸（NADPH）-细胞色素 P450 还原酶（CPR）是 P450 单加氧酶催化的各种生物反应所必需的电子转移伴侣，我们之前的研究表明，增强的 P450 酶解毒和 CYP6ER1 过表达有助于褐飞虱对硫氟肟的抗性。然而，CPR、硫氟肟抗性和新烟碱类交叉抗性之间的关系在褐飞虱中仍不清楚。在本研究中，建立了具有相同遗传背景的褐飞虱硫氟肟抗性（SFX-SEL）（RR=254.04 倍）、抗性衰退（DESEL）（RR=18.99 倍）和敏感未选择（UNSEL）品系。实时定量聚合酶链反应（RT-qPCR）显示，SFX-SEL 品系的褐飞虱 CPR（NlCPR）表达水平分别比 UNSEL 和 DESEL 品系高 6.85 倍和 6.07 倍。NlCPR 在 UNSEL、DESEL 和 SFX-SEL 四龄若虫的腹部表达水平明显高于其他组织（胸部、头部和腿部）。此外，硫氟肟胁迫显著增加了 UNSEL、SFX-SEL 和 DESEL 品系中 NlCPR mRNA 水平。RNA 干扰（RNAi）沉默 NlCPR 显著增加了 UNSEL、DESEL 和 SFX-SEL 品系对硫氟肟的敏感性，但 SFX-SEL 的恢复更为明显。此外，NlCPR 沉默导致所有品系（UNSEL、DESEL 和 SFX-SEL）对吡虫啉、噻虫嗪、噻虫胺和噻虫啉的敏感性显著恢复，其中对硫氟肟抗性品系（SFX-SEL）的恢复程度最大。我们的研究结果表明，NlCPR 过表达有助于褐飞虱对硫氟肟的抗性和新烟碱类交叉抗性。这将有助于阐明 CPR 在褐飞虱 P450 介导的代谢抗性进化中的意义。
