Hey Daniel, Grimm Bernhard
a Lebenswissenschaftliche Fakultät, Institut für Biologie, AG Pflanzenphysiologie , Humboldt-Universität zu Berlin , Berlin , Germany.
Plant Signal Behav. 2018;13(12):e1550317. doi: 10.1080/15592324.2018.1550317. Epub 2018 Nov 29.
Plant ONE-HELIX PROTEINS (OHPs) are part of the light-harvesting complex superfamily whose members are involved in various processes related to sensing and capturing light as well as light protection. We recently showed the requirement of a functional OHP1-OHP2 heterodimer for efficient D1 synthesis. Interestingly, while the knockout mutant showed a strong defect in accumulation of the photosystem II and is hardly viable, virus-induced gene silencing of had no detectable impact on plant growth and performance under standard growth conditions. However, labeling assays with S-methionine indicate a reduced D1 synthesis rate. Here, we show that VIGS-OHP1 plants are more susceptible towards elevated light intensities than control plants. This underlines an obligatory function of OHP1 for light acclimation.
植物单螺旋蛋白（OHPs）是捕光复合体超家族的一部分，其成员参与了与感知和捕获光以及光保护相关的各种过程。我们最近表明，功能性的OHP1-OHP2异二聚体对于高效合成D1是必需的。有趣的是，虽然敲除突变体在光系统II的积累方面表现出严重缺陷且几乎无法存活，但在标准生长条件下，病毒诱导的基因沉默对植物生长和性能没有可检测到的影响。然而，用S-甲硫氨酸进行的标记试验表明D1合成速率降低。在这里，我们表明VIGS-OHP1植物比对照植物对高光强度更敏感。这突出了OHP1在光适应中的重要作用。
