Li Ying-Xiu, Wang Nan-Nan, Chen Guan-Jun, Du Zong-Jun
1College of Marine Science, Shandong University, Weihai 264209, PR China.
2State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2019 Jan;69(1):255-260. doi: 10.1099/ijsem.0.003146. Epub 2018 Nov 29.
A novel Gram-stain-negative, rod-shaped, ivory-white, facultatively anaerobic and catalase-positive bacterium, designated H1304, was isolated from the gut of sea catfish from Coast of Weihai, China. Optimal growth occurred at 30-33 °C (range, 4-37 °C) and pH 7.0-7.5 (range, pH 6.5-9.0) with 2.0-3.0 % (w/v) NaCl (range, 0.5-4.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that H1304 belonged to the genus Cohaesibacter and was most closely related to Cohaesibactermarisflavi CGMCC 1.9157 (96.7 % 16S rRNA gene sequence similarity), Cohaesibactergelatinilyticus MCCC 1A02698 (96.3 %) and Cohaesibacterhaloalkalitolerans KCTC 32038 (96.0 %). The sole isoprenoid quinone was Q-10, the polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylcholine, glycolipid, an unidentified phospholipid and an unidentified aminolipid. The major fatty acids (>10 %) were C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The DNA G+C content of strain H1304 is 50.8 mol%. Based on the combination of phylogenetic analysis, phenotypic data and chemotaxonomic data, strain H1304 is considered to represent a novel species within the genus Cohaesibacter in the family Cohaesibacteraceae, for which the name Cohaesibacter celericrescens sp. nov. is proposed. The type strain of the new species is H1304 (=KCTC 62075=MCCC 1H00241).
从中国威海海岸海鲶鱼肠道中分离出一株革兰氏染色阴性、杆状、象牙白色、兼性厌氧且过氧化氢酶阳性的新型细菌，命名为H1304。其最适生长温度为30 - 33°C（范围为4 - 37°C），最适pH为7.0 - 7.5（范围为pH 6.5 - 9.0），NaCl浓度为2.0 - 3.0%（w/v）（范围为0.5 - 4.0%）。基于16S rRNA基因序列的系统发育分析表明，H1304属于Cohaesibacter属，与海黄Cohaesibacter marisflavi CGMCC 1.9157（16S rRNA基因序列相似性为96.7%）、溶明胶Cohaesibacter gelatinilyticus MCCC 1A02698（96.3%）和耐盐碱Cohaesibacter haloalkalitolerans KCTC 32038（96.0%）关系最为密切。唯一的类异戊二烯醌为Q - 10，极性脂质谱由磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、磷脂酰胆碱、糖脂、一种未鉴定的磷脂和一种未鉴定的氨基脂质组成。主要脂肪酸（>10%）为C18 : 1ω7c和总和特征3（C16 : 1ω7c/C16 : 1ω6c）。菌株H1304的DNA G + C含量为50.8 mol%。基于系统发育分析、表型数据和化学分类数据的综合结果，菌株H1304被认为代表Cohaesibacteraceae科Cohaesibacter属内的一个新物种，为此提出新名称Cohaesibacter celericrescens sp. nov.。该新物种的模式菌株为H1304（=KCTC 62075 = MCCC 1H00241）。
