Zhang H-L, Jia F, Li M, Yu F, Zhou B, Hao Q-H, Wang X-L
College of Life Sciences, Agricultural University of Hebei, Baoding, China.
Lett Appl Microbiol. 2019 Mar;68(3):226-233. doi: 10.1111/lam.13102. Epub 2019 Jan 20.
Medicago sativa L. (alfalfa) is an important forage crop throughout the world. Despite the abiotic nutritional components of alfalfa having been extensively studied, there is only limited information on alfalfa endophytes. In this study, thirteen endophytic bacteria were isolated from alfalfa seeds. Bacillus (76·9%) was the most abundant genus, followed by Enterobacter (15·4%), Brevibacterium (7·7%), Geobacillus (7·7%) and Staphylococcus (7·7%). Four of the 13 endophytic bacteria, including Bacillus amyloliquefaciens EnB-alf1, Bacillus subtilis EnB-alf3, EnB-alf5 and EnB-alf13, were capable of significantly extending the lifespan of Caenorhabditis elegans. In addition, B. amyloliquefaciens EnB-alf1 enhanced the resistance of C. elegans to thermal stress whereas B. subtilis EnB-alf3 enhanced the resistance to oxidative stress. Further studies demonstrated that the enhanced lifespan of the worm was depended on the function of DAF-2/DAF-16 and was associated with the colonization of strain in the worms' intestines when strain EnB-alf1 or strain EnB-alf3 was presented to the worms as food sources. Our results suggest that some endophytic Bacillus strains isolated from alfalfa are beneficial on C. elegans health. SIGNIFICANCE AND IMPACT OF THE STUDY: Medicago sativa L. (alfalfa) is an important forage crop throughout the world. Despite the abiotic nutritional components of alfalfa having been extensively studied, there is only limited information available on alfalfa endophytes. Beneficial bacteria residing in the host intestine have been shown to affect host longevity. However, there is limited information available on the functions of alfalfa seed endophytes to nematodes. In this study, four endophytic Bacillus strains isolated from alfalfa seeds were found to significantly extend the lifespan of Caenorhabditis elegans and enhance resistance to thermal and oxidative stress. Our results suggest that some endophytic Bacillus strains isolated from alfalfa seeds can promote good health in C. elegans.
紫花苜蓿（Medicago sativa L.）是一种在全球范围内重要的饲料作物。尽管紫花苜蓿的非生物营养成分已得到广泛研究，但关于紫花苜蓿内生菌的信息却十分有限。在本研究中，从紫花苜蓿种子中分离出了13种内生细菌。芽孢杆菌属（76.9%）是最主要的菌属，其次是肠杆菌属（15.4%）、短杆菌属（7.7%）、嗜热栖热菌属（7.7%）和葡萄球菌属（7.7%）。13种内生细菌中的4种，包括解淀粉芽孢杆菌EnB-alf1、枯草芽孢杆菌EnB-alf3、EnB-alf5和EnB-alf13，能够显著延长秀丽隐杆线虫的寿命。此外，解淀粉芽孢杆菌EnB-alf1增强了秀丽隐杆线虫对热应激的抗性，而枯草芽孢杆菌EnB-alf3增强了其对氧化应激的抗性。进一步研究表明，线虫寿命的延长依赖于DAF-2/DAF-16的功能，并且当将菌株EnB-alf1或菌株EnB-alf3作为食物来源提供给线虫时，与菌株在虫体肠道中的定殖有关。我们的结果表明，从紫花苜蓿中分离出的一些内生芽孢杆菌菌株对秀丽隐杆线虫的健康有益。研究的意义和影响：紫花苜蓿（Medicago sativa L.）是一种在全球范围内重要的饲料作物。尽管紫花苜蓿的非生物营养成分已得到广泛研究，但关于紫花苜蓿内生菌的可用信息却十分有限。已表明存在于宿主肠道中的有益细菌会影响宿主寿命。然而，关于紫花苜蓿种子内生菌对线虫功能的可用信息却很有限。在本研究中，发现从紫花苜蓿种子中分离出的4种内生芽孢杆菌菌株能显著延长秀丽隐杆线虫的寿命，并增强其对热应激和氧化应激的抗性。我们的结果表明，从紫花苜蓿种子中分离出的一些内生芽孢杆菌菌株可促进秀丽隐杆线虫的健康。
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