Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Rosario 2000, Argentina.
Nat Commun. 2017 Jan 30;8:14332. doi: 10.1038/ncomms14332.
Beneficial bacteria have been shown to affect host longevity, but the molecular mechanisms mediating such effects remain largely unclear. Here we show that formation of Bacillus subtilis biofilms increases Caenorhabditis elegans lifespan. Biofilm-proficient B. subtilis colonizes the C. elegans gut and extends worm lifespan more than biofilm-deficient isogenic strains. Two molecules produced by B. subtilis - the quorum-sensing pentapeptide CSF and nitric oxide (NO) - are sufficient to extend C. elegans longevity. When B. subtilis is cultured under biofilm-supporting conditions, the synthesis of NO and CSF is increased in comparison with their production under planktonic growth conditions. We further show that the prolongevity effect of B. subtilis biofilms depends on the DAF-2/DAF-16/HSF-1 signalling axis and the downregulation of the insulin-like signalling (ILS) pathway.
有益细菌已被证明会影响宿主的寿命，但介导这种影响的分子机制在很大程度上仍不清楚。在这里，我们表明枯草芽孢杆菌生物膜的形成会延长秀丽隐杆线虫的寿命。能够形成生物膜的枯草芽孢杆菌会定殖于秀丽隐杆线虫的肠道，并比不能形成生物膜的同基因缺陷菌株延长蠕虫的寿命。枯草芽孢杆菌产生的两种分子 - 群体感应五肽 CSF 和一氧化氮（NO） - 足以延长秀丽隐杆线虫的寿命。当枯草芽孢杆菌在支持生物膜形成的条件下培养时，与在浮游生长条件下相比，其合成 NO 和 CSF 的量会增加。我们进一步表明，枯草芽孢杆菌生物膜的延长寿命效应取决于 DAF-2/DAF-16/HSF-1 信号通路和胰岛素样信号（ILS）通路的下调。
