da Silva Priscila D A, Uscategui Ricardo Andrés R, Santos Victor J C, Taira Augusto R, Mariano Renata S G, Rodrigues Mariana G K, Simões Ana Paula R, Maronezi Marjury C, Avante Michelle L, Monteiro Frederico O B, Vicente Wilter R R, Feliciano Marcus A R
Faculda de Ciências Agrárias e Veterinárias, Department of Animal Reproduction, Universidade Estadual Paulista, Jaboticabal, Brazil.
Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia, Belem, Brazil.
Reprod Domest Anim. 2019 Mar;54(3):498-505. doi: 10.1111/rda.13384. Epub 2018 Dec 15.
The aim of this study was to evaluate the stiffness of foetal lungs, liver, kidneys and placentomes by acoustic radiation force impulse (ARFI) elastography in ovine and to correlate obtained findings with foetal development. Twenty-four ewes were included, and tissue stiffness of foetal lung, liver, kidney and placentome was measured beginning with 10th gestational week by qualitative-quantitative ARFI elastography. A total of 33 healthy lambs were born. Qualitative elastographic analysis permitted to classify maternal and foetal tissues elasticity in decreasing order as follows: placentome, kidney, liver and lung. Regarding quantitative ARFI elastography data, shear wave velocity (SWV) of foetal lung and liver varied. The lung SWV decreased gradually from the 16th to the 21st gestational week (R = 0.80; p < 0.001), while liver SWV increased gradually from the 14th to 21st gestational week (R = 0.80; p < 0.001). In contrast, the kidneys and placentomes SWV's remained constant through gestation (p = 0.076; and 0.34). ARFI elastography was shown feasible for evaluation of maternal and foetal tissues stiffness in the ovine model. It can be used to demonstrate pulmonary and hepatic stiffness modifications during foetal development.
本研究旨在通过声辐射力脉冲（ARFI）弹性成像技术评估绵羊胎儿肺、肝、肾和胎盘叶的硬度，并将所得结果与胎儿发育情况相关联。纳入了24只母羊，从妊娠第10周开始，通过定性-定量ARFI弹性成像技术测量胎儿肺、肝、肾和胎盘叶的组织硬度。共出生33只健康羔羊。定性弹性成像分析可将母体和胎儿组织的弹性按降序分类如下：胎盘叶、肾、肝和肺。关于定量ARFI弹性成像数据，胎儿肺和肝的剪切波速度（SWV）有所不同。肺SWV从妊娠第16周到21周逐渐降低（R = 0.80；p < 0.001），而肝SWV从妊娠第14周到21周逐渐升高（R = 0.80；p < 0.001）。相比之下，肾和胎盘叶的SWV在整个妊娠期保持恒定（p = 0.076；和0.34）。ARFI弹性成像技术在绵羊模型中被证明可用于评估母体和胎儿组织的硬度。它可用于展示胎儿发育过程中肺和肝硬度的变化。
