Department of Animal Reproduction, Faculty of Agricultural and Veterinary Sciences, Univ. Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, São Paulo, Brazil.
Department of Veterinary Surgery, Faculty of Agricultural and Veterinary Sciences, Univ. Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, São Paulo, Brazil.
Anim Reprod Sci. 2020 Mar;214:106289. doi: 10.1016/j.anireprosci.2020.106289. Epub 2020 Feb 5.
Placental rigidity and biometry of twelve pregnant bitches were evaluated using B-mode and Acoustic Radiation Force Impulse (ARFI) ultrasonography, performed once daily, from day 15 of gestation until parturition. Specific software (Virtual Touch Tissue Quantification® VTTQ and Virtual Touch Tissue Imaging Quantification® VTTIQ) were used. Values for results for variables were correlated and regression models related to gestational day were used to make evaluations. Maternal-fetal placental thickness increased to day 63 (P < 0.0001; R² = 0.91); maternal placental thickness increased until day 40 (P = 0.0340; R² = 0.54); and fetal placental thickness increased to day 50 (P < 0.0001; R² = 0.83) of gestation. Shear wave velocity (SWV) of the dorsal (P < 0.0010) was greater than lateral, which in turn was greater (P = 0.020) than the ventral area. The SWV of the dorsal area as determined using VTTQ, decreased from day 21-35 and increased to day 56 of gestation (P = 0.0291; R² = 0.4021); lateral SWV decreased from day 24-45 and increased until the time of parturition (P < 0.001; R² = 0.6055). The SWV of the dorsal area, as determined using VTTIQ, decreased from day 21-43 and then increased to day 60 of gestation (P = 0.0016; R² = 0.5075); and ventral area SWV increased from day 21-23 and decreased until the time of parturition (P < 0.001; R² = 0.8055). Placental alterations reflect structural and biochemical gestational adaptations and can become useful techniques for obstetrics.
采用 B 型和声辐射力脉冲（ARFI）超声技术，每天一次，对 12 只怀孕母犬的胎盘刚性和生物计量进行评估，从妊娠第 15 天开始，直到分娩。使用特定的软件（虚拟触摸组织定量技术 VTTQ 和虚拟触摸组织成像定量技术 VTTIQ）。对变量的结果值进行相关性分析，并使用与妊娠天数相关的回归模型进行评估。母体-胎儿胎盘厚度增加至第 63 天（P<0.0001；R²=0.91）；母体胎盘厚度增加至第 40 天（P=0.0340；R²=0.54）；胎儿胎盘厚度增加至第 50 天（P<0.0001；R²=0.83）。背侧的剪切波速度（SWV）（P<0.0010）大于侧部，而侧部又大于（P=0.020）腹侧。使用 VTTQ 测量的背侧区域的 SWV，从第 21-35 天开始下降，在第 56 天妊娠时增加（P=0.0291；R²=0.4021）；侧部 SWV 从第 24-45 天开始下降，一直增加到分娩时（P<0.001；R²=0.6055）。使用 VTTIQ 测量的背侧区域的 SWV，从第 21-43 天下降，然后在第 60 天妊娠时增加（P=0.0016；R²=0.5075）；腹侧区域的 SWV 从第 21-23 天增加，然后在分娩时下降（P<0.001；R²=0.8055）。胎盘的变化反映了妊娠的结构和生化适应，可以成为产科的有用技术。
