肿瘤坏死因子和淋巴毒素的两个保守色氨酸残基不参与生物活性。
Two conserved tryptophan residues of tumor necrosis factor and lymphotoxin are not involved in the biological activity.
作者信息
Van Ostade X, Tavernier J, Fiers W
机构信息
Laboratory of Molecular Biology, State University of Ghent, Belgium.
出版信息
FEBS Lett. 1988 Oct 10;238(2):347-52. doi: 10.1016/0014-5793(88)80510-6.
PMID:3049162
Abstract
Each of the two highly conserved tryptophan residues in hTNF (positions 28 and 114) was converted into phenylalanine by site-directed mutagenesis and the mutant proteins were partially purified. A cytotoxicity assay on mouse L929 cells showed only a slight reduction in biological activity, strongly suggesting that neither of the two amino acids is involved in the active site.
摘要
通过定点诱变将人肿瘤坏死因子（hTNF）中两个高度保守的色氨酸残基（第28位和第114位）中的每一个都转化为苯丙氨酸，并对突变蛋白进行了部分纯化。对小鼠L929细胞的细胞毒性试验表明，其生物活性仅略有降低，这有力地表明这两个氨基酸均不参与活性位点。
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