West P A
Aquatic Environment Protection Division 2, Ministry of Agriculture, Fisheries and Food, Burnham-on-Crouch, Essex, UK.
J Appl Bacteriol. 1988 Apr;64(4):345-53. doi: 10.1111/j.1365-2672.1988.tb01879.x.
An improved membrane filtration procedure for use on board ship to enumerate Escherichia coli and Group D faecal streptococci in marine sediments is described. Ultrasonication extraction combined with resuscitation of sublethally-injured cells yielded significantly higher counts of E. coli than sediments shaken by hand. Counts of E. coli were also higher on mFC agar (without rosalic acid) after a period of resuscitation on tryptone-soy agar supplemented with 0.1% yeast extract than on a 4% Teepol-lactose medium. Ultrasonication of sediments made no significant difference to counts of Group D faecal streptococci on KF-streptococcus agar. These improved isolation procedures allowed better discrimination of the area affected by sewage sludge at a disposal site off the northeast coast of England.
本文描述了一种改良的膜过滤程序，用于在船上对海洋沉积物中的大肠杆菌和D群粪链球菌进行计数。超声提取结合亚致死损伤细胞的复苏，所得到的大肠杆菌计数显著高于手动振荡的沉积物。在补充有0.1%酵母提取物的胰蛋白胨大豆琼脂上复苏一段时间后，在mFC琼脂（不含玫瑰红酸）上的大肠杆菌计数也高于在4%吐温-80乳糖培养基上的计数。沉积物的超声处理对KF-链球菌琼脂上D群粪链球菌的计数没有显著影响。这些改良的分离程序能够更好地辨别英格兰东北海岸一处处置场受污水污泥影响的区域。
