Lappin-Scott H M, Cusack F, MacLeod A, Costerton J W
Department of Biological Sciences, University of Calgary, Alberta, Canada.
J Appl Bacteriol. 1988 Jun;64(6):541-9. doi: 10.1111/j.1365-2672.1988.tb02445.x.
Klebsiella pneumoniae isolated from oil well waters reduced in size in response to nutrient starvation. The cells remained viable during starvation and later were able to grow rapidly when stimulated by nutrients. The heterotrophic potential, culture absorbance and extracellular polysaccharide production decreased during cell starvation whereas an initial increase in colony-forming units was observed on agar plates. Transmission electron microscopy (TEM) after 24 d revealed that the cells had changed to small rods or cocci between 0.5 by 0.25 micron and 0.87 by 0.55 micron. When transferred to half-strength brain heart infusion medium, TEM showed cell division and rod-shaped cells after 45 min and full resuscitation within 4 h. Cell response was much slower in sodium citrate medium and resuscitation took 8 h.
从油井水中分离出的肺炎克雷伯菌在营养饥饿时体积减小。细胞在饥饿期间仍保持活力，之后在营养刺激下能够迅速生长。细胞饥饿期间，其异养潜力、培养物吸光度和胞外多糖产量均下降，而在琼脂平板上观察到菌落形成单位最初有所增加。24天后的透射电子显微镜（TEM）显示，细胞已变为大小在0.5×0.25微米至0.87×0.55微米之间的小杆菌或球菌。转移至半强度脑心浸液培养基后，TEM显示45分钟后细胞开始分裂并出现杆状细胞，4小时内完全复苏。在柠檬酸钠培养基中细胞反应要慢得多，复苏需要8小时。
