在大肠杆菌中表达的类胡萝卜素克隆基因在抵御近紫外光和特定光毒性分子失活方面的作用。
Role of cloned carotenoid genes expressed in Escherichia coli in protecting against inactivation by near-UV light and specific phototoxic molecules.
作者信息
Tuveson R W, Larson R A, Kagan J
机构信息
Department of Microbiology, University of Illinois, Urbana-Champaign 61801.
出版信息
J Bacteriol. 1988 Oct;170(10):4675-80. doi: 10.1128/jb.170.10.4675-4680.1988.
Abstract
Genes controlling carotenoid synthesis were cloned from Erwinia herbicola and expressed in an Escherichia coli strain. Carotenoids protect against high fluences of near-UV (NUV; 320 to 400 nm) but not against far-UV (200-300 nm). Protection of E. coli cells was not observed following treatment with either psoralen or 8-methoxypsoralen plus NUV. However, significant protection of cells producing carotenoids was observed with three photosensitizing molecules activated by NUV (alpha-terthienyl, harmine, and phenylheptatriyne) which are thought to have the membrane as an important lethal target. Protection of carotenoid-producing cells against inactivation was not observed with acridine orange plus visible light but was seen with toluidine blue O plus visible light.
摘要
从草生欧文氏菌中克隆出控制类胡萝卜素合成的基因，并在大肠杆菌菌株中表达。类胡萝卜素可抵御高强度的近紫外线（NUV；320至400纳米），但不能抵御远紫外线（200 - 300纳米）。用补骨脂素或8 - 甲氧基补骨脂素加近紫外线处理后，未观察到对大肠杆菌细胞的保护作用。然而，在用近紫外线激活的三种光敏分子（α-三联噻吩、哈尔明和苯基庚三炔）处理时，观察到对产生类胡萝卜素的细胞有显著保护作用，这些光敏分子被认为以细胞膜作为重要的致死靶点。用吖啶橙加可见光处理时，未观察到对产生类胡萝卜素的细胞的失活保护作用，但用甲苯胺蓝O加可见光处理时则观察到了这种保护作用。
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