Wakayama Y, Takagi M, Yano K
J Bacteriol. 1984 Aug;159(2):527-32. doi: 10.1128/jb.159.2.527-532.1984.
Escherichia coli cells were killed by visible light irradiation in the presence of the photosensitizing dye, toluidine blue. Two uvrB mutant strains of E. coli K-12 (AB1885 and N3-1) were much more sensitive than the isogenic uvrA and uvrC strains to treatment with toluidine blue plus light, suggesting that the uvrB+ gene product was involved in repair of DNA damage induced by the treatment. The uvrB+ gene cloned in a high- or low-copy-number plasmid was transformed into the uvrB strain (AB1885). Although all the transformants showed the same resistance as its wild-type strain (AB1157) to UV irradiation, they were as sensitive as AB1885 was to treatment with toluidine blue plus light. The two uvrB strains were more sensitive to sodium dodecyl sulfate than the other strains, suggesting that these strains had a defect in the cell surface. A sodium dodecyl sulfate-resistant revertant obtained from AB1885 was more resistant than AB1885 was to treatment with toluidine blue plus light. The two uvrB strains (AB1885 and N3-1) appear to have a defective gene (tentatively called dvl) different from uvrB. Its map position was around 7 min on the E. coli map.
在光敏染料甲苯胺蓝存在的情况下，大肠杆菌细胞经可见光照射后被杀死。大肠杆菌K-12的两个uvrB突变株（AB1885和N3-1）对甲苯胺蓝加光处理的敏感性比同基因的uvrA和uvrC菌株高得多，这表明uvrB⁺基因产物参与了该处理诱导的DNA损伤修复。将克隆在高拷贝或低拷贝质粒中的uvrB⁺基因转化到uvrB菌株（AB1885）中。虽然所有转化体对紫外线照射的抗性与其野生型菌株（AB1157）相同，但它们对甲苯胺蓝加光处理的敏感性与AB1885一样。这两个uvrB菌株对十二烷基硫酸钠比其他菌株更敏感，表明这些菌株在细胞表面存在缺陷。从AB1885获得的一个耐十二烷基硫酸钠的回复突变体对甲苯胺蓝加光处理的抗性比AB1885更高。这两个uvrB菌株（AB1885和N3-1）似乎有一个与uvrB不同的缺陷基因（暂称为dvl）。其在大肠杆菌染色体图谱上的定位约在7分钟处。
