Key Laboratory of Crop Eco-physiology and Farming System in Southwest China (Ministry of Agriculture), College of Agronomy, Sichuan Agricultural University, 211 Huimin Road, Wenjiang district, Chengdu, China.
J Biochem. 2019 Mar 1;165(3):277-288. doi: 10.1093/jb/mvy103.
Soybean-based intercropping is important for sustainable agricultural practice on a regional and global scale. However, most soybean varieties use shade avoidance strategy to acquire more light absorption when suffered in canopy shade in intercropping systems, thus reduced the yield of the whole population on a farmland. The mechanisms underlying early response of soybean in shade avoidance is still largely unknown. Here we report our identification of differentially accumulated proteins in shade-sensitive soybean seedlings by global quantitative proteome analysis under white light (WL) and shade conditions. By using Tandem Mass Tag (TMT) labelling and HPLC fractionation followed by high-resolution LC-MS/MS analysis, 29 proteins were found up-regulated and 412 proteins were found down-regulated in soybean seedlings by 2-h shade stress than that by 2-h WL treatment. Multiple differentially expressed proteins are enriched in carbohydrate metabolic process especially in the biosynthetic pathways of cell wall polysaccharides in soybean seedlings by shade stress comparing to those in WL growth conditions. Physiological assays showed that saccharides were rapidly declined in shoot apex of soybean seedlings under a short-term shading. Our results would provide new insights into the mechanisms of shade avoidance responses in soybean seedlings.
大豆间作在区域和全球范围内对于可持续农业实践非常重要。然而，大多数大豆品种在间作系统中遭受冠层遮荫时会采用避荫策略来获取更多的光吸收，从而降低农田上整个种群的产量。大豆在避荫早期响应的机制在很大程度上仍然未知。在这里，我们通过在白光 (WL) 和遮荫条件下进行全局定量蛋白质组分析，报告了我们对遮荫敏感的大豆幼苗中差异积累蛋白的鉴定。通过使用串联质量标签 (TMT) 标记和 HPLC 分级，然后进行高分辨率 LC-MS/MS 分析，发现与 WL 生长条件相比，在 2 小时遮荫胁迫下，大豆幼苗中有 29 个蛋白上调，412 个蛋白下调。与 WL 生长条件相比，在遮荫胁迫下，大豆幼苗中多个差异表达蛋白富集在碳水化合物代谢过程中，特别是在细胞壁多糖的生物合成途径中。生理测定表明，在短期遮荫下，大豆幼苗的茎尖中糖迅速下降。我们的研究结果将为大豆幼苗避荫反应的机制提供新的见解。
