College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Int J Mol Sci. 2023 Jan 5;24(2):1017. doi: 10.3390/ijms24021017.
Soybean stem elongation and thickening are related to cell wall composition. Plant morphogenesis can be influenced by blue light, which can regulate cell wall structure and composition, and affect stem growth and development. Here, using proteomics and metabolomics, differentially expressed proteins and metabolites of hypocotyls grown in the dark and under blue light were studied to clarify the effects of blue light on the cell wall structure and carbohydrate metabolism pathway of soybean hypocotyls. Results showed that 1120 differential proteins were upregulated and 797 differential proteins were downregulated under blue light treatment, while 63 differential metabolites were upregulated and 36 differential metabolites were downregulated. Blue light promoted the establishment of cell wall structure and composition by regulating the expression of both the enzymes and metabolites related to cell wall structural composition and nonstructural carbohydrates. Thus, under blue light, the cross-sectional area of the hypocotyl and xylem were larger, the longitudinal length of pith cells was smaller, elongation of the soybean hypocotyl was inhibited, and diameter was increased.
大豆茎的伸长和加粗与细胞壁组成有关。植物形态发生可以受到蓝光的影响，蓝光可以调节细胞壁结构和组成，影响茎的生长和发育。在这里，使用蛋白质组学和代谢组学，研究了在黑暗中和蓝光下生长的下胚轴的差异表达蛋白和代谢物，以阐明蓝光对大豆下胚轴细胞壁结构和碳水化合物代谢途径的影响。结果表明，蓝光处理下有 1120 个差异蛋白上调，797 个差异蛋白下调，同时有 63 个差异代谢物上调，36 个差异代谢物下调。蓝光通过调节与细胞壁结构组成和非结构性碳水化合物相关的酶和代谢物的表达，促进细胞壁结构和组成的建立。因此，在蓝光下，下胚轴和木质部的横截面积更大，髓细胞的纵向长度更小，大豆下胚轴的伸长受到抑制，直径增加。
