Jia Qianru, Hu Shengyan, Li Xihuan, Wei Libin, Wang Qiong, Zhang Wei, Zhang Hongmei, Liu Xiaoqing, Chen Xin, Wang Xuejun, Chen Huatao
Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong, China.
Front Plant Sci. 2024 Sep 27;15:1479536. doi: 10.3389/fpls.2024.1479536. eCollection 2024.
Shade has a direct impact on photosynthesis and production of plants. Exposure to shade significantly reduces crops yields. Identifying shade-tolerant genomic loci and soybean varieties is crucial for improving soybean yields. In this study, we applied a shade treatment (30% light reduction) to a natural soybean population consisting of 264 accessions, and measured several traits, including the first pod height, plant height, pod number per plant, grain weight per plant, branch number, and main stem node number. Additionally, we performed GWAS on these six traits with and without shade treatment, as well as on the shade tolerance coefficients (STCs) of the six traits. As a result, we identified five shade-tolerance varieties, 733 SNPs and four candidate genes over two years. Furthermore, we developed four kompetitive allele-specific PCR (KASP) makers for the STC of S18_1766721, S09_48870909, S19_49517336, S18_3429732. This study provides valuable genetic resources for breeding soybean shade tolerance and offers new insights into the theoretical research on soybean shade tolerance.
遮荫对植物的光合作用和产量有直接影响。暴露于遮荫环境会显著降低作物产量。鉴定耐荫基因组位点和大豆品种对于提高大豆产量至关重要。在本研究中，我们对由264份材料组成的天然大豆群体进行了遮荫处理（光照减少30%），并测量了几个性状，包括第一节荚高度、株高、单株荚数、单株粒重、分枝数和主茎节数。此外，我们对这六个性状在有和没有遮荫处理的情况下以及对这六个性状的耐荫系数（STC）进行了全基因组关联研究（GWAS）。结果，我们在两年内鉴定出了五个耐荫品种、733个单核苷酸多态性（SNP）和四个候选基因。此外，我们针对S18_1766721、S09_48870909、S19_49517336、S18_3429732的耐荫系数开发了四个竞争性等位基因特异性PCR（KASP）标记。本研究为大豆耐荫育种提供了宝贵的遗传资源，并为大豆耐荫性的理论研究提供了新的见解。
