School of Chemistry and Materials Science, Heilongjiang University, 74 Xuefu Road, Harbin 150080, PR China.
School of Chemistry and Materials Science, Heilongjiang University, 74 Xuefu Road, Harbin 150080, PR China.
Colloids Surf B Biointerfaces. 2019 Feb 1;174:476-482. doi: 10.1016/j.colsurfb.2018.11.043. Epub 2018 Nov 20.
The chemical stability, good biocompatibility and high drug loading capacity of zinc oxide nanoparticles (ZnO NPs) and their biomedical potentials make them a promising candidate for drug delivery. The aim of this study was to develop and assess a simple procedure for surface functionalization of ZnO NPs by N-acetyl-l-cysteine (NAC) for anticancer camptothecin (CPT) delivery. NAC capped ZnO NPs were successfully made using ZnCl and NaOH in the presence of NAC. CPT was covalently conjugated to the surface of as-synthesized ZnO-NAC NPs. To characterize the synthesized conjugate product (ZnO-NAC-CPT NPs), X-ray diffraction, Fourier Transform Infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, and dynamic light scattering method were used. Our results indicated that the ZnO-NAC-CPT NPs exhibit near-spherical morphology and uniform dispersion with an average diameter of ∼70 nm. The hemolysis assay showed that ZnO-NAC-CPT NPs has almost no hemolytic activity. In addition, MTT cytotoxicity assessment on A549 lung cancer cells revealed a drop of IC values from 1.17 μg/mL (free CPT) to 0.66 μg/mL (ZnO-NAC-CPT NPs). This result showed an augmented cancer-inhibitory effect of nanoconjugate complex. In conclusion, the novel ZnO-NAC-CPT NPs could be considered for new therapeutic endeavors.
氧化锌纳米粒子（ZnO NPs）具有化学稳定性、良好的生物相容性和高载药能力，以及它们在生物医学方面的潜力，使它们成为药物输送的有前途的候选物。本研究旨在开发和评估一种通过 N-乙酰-L-半胱氨酸（NAC）对 ZnO NPs 进行表面功能化的简单方法，用于抗癌喜树碱（CPT）的递送。在 NAC 的存在下，使用 ZnCl 和 NaOH 成功地制备了 NAC 封端的 ZnO NPs。CPT 通过共价键连接到合成的 ZnO-NAC NPs 表面。为了表征合成的轭合物产物（ZnO-NAC-CPT NPs），使用了 X 射线衍射、傅里叶变换红外光谱、透射电子显微镜、扫描电子显微镜和动态光散射法。我们的结果表明，ZnO-NAC-CPT NPs 呈近球形形态，具有均匀的分散性，平均直径约为 70nm。溶血试验表明 ZnO-NAC-CPT NPs 几乎没有溶血活性。此外，对 A549 肺癌细胞的 MTT 细胞毒性评估表明，IC 值从游离 CPT 的 1.17μg/mL 降至 ZnO-NAC-CPT NPs 的 0.66μg/mL。该结果表明纳米缀合物复合物具有增强的抗癌作用。总之，新型 ZnO-NAC-CPT NPs 可用于新的治疗方法。
