Ghaffari Seyed-Behnam, Sarrafzadeh Mohammad-Hossein, Fakhroueian Zahra, Shahriari Shadab, Khorramizadeh M Reza
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Oct 1;79:465-472. doi: 10.1016/j.msec.2017.05.065. Epub 2017 May 11.
Inherent biocompatibility and stability of zinc oxide nanoparticles (ZnO-NPs) and their biomedical potentials make them an emerging candidate for drug delivery. The aim of this study was to develop and assess a simple procedure for surface functionalization of ZnO-NPs by 3-mercaptopropionic acid (MPA) for water-soluble curcumin delivery. Carboxyl-terminated ZnO nanoparticles were successfully made using ZnCl and NaOH in the presence of MPA. The functional groups were activated by 1,1'-carbonyldiimidazole (CDI) and the curcumin bonding was carried out at room temperature for 24h. The core-shell nanocomposite had a significant better solubility versus free curcumin, as characterized by XRD, FTIR, UV-Vis spectrophotometry, DLS, and TEM, p<0.005. In addition, MTT cytotoxicity assessment on MDA-MB-231 breast cancer cells revealed a drop of IC values from 5μg/mL to 3.3μg/mL for free curcumin and ZnO-MPA-curcumin complex, respectively. This result showed an augmented cancer-inhibitory effect of nanoconjugate complex. In conclusion, the presented improved solubility and elevated functionality of novel ZnO-MPA-curcumin nanoformula is promising, and could be considered for new therapeutic endeavors.
氧化锌纳米颗粒（ZnO-NPs）固有的生物相容性和稳定性及其生物医学潜力使其成为药物递送的新兴候选者。本研究的目的是开发并评估一种通过3-巯基丙酸（MPA）对ZnO-NPs进行表面功能化以实现水溶性姜黄素递送的简单程序。在MPA存在的情况下，使用ZnCl和NaOH成功制备了羧基封端的ZnO纳米颗粒。官能团通过1,1'-羰基二咪唑（CDI）活化，姜黄素键合在室温下进行24小时。通过XRD、FTIR、紫外可见分光光度法、动态光散射（DLS）和透射电子显微镜（TEM）表征，核壳纳米复合材料的溶解度相对于游离姜黄素显著提高，p<0.005。此外，对MDA-MB-231乳腺癌细胞的MTT细胞毒性评估显示，游离姜黄素和ZnO-MPA-姜黄素复合物的IC值分别从5μg/mL降至3.3μg/mL。该结果表明纳米共轭复合物的癌症抑制作用增强。总之，新型ZnO-MPA-姜黄素纳米制剂所呈现出的改善的溶解度和增强的功能很有前景，可考虑用于新的治疗尝试。
