Division of Environmental and Biomolecular Systems, Institute of Environmental Health, Oregon Health & Science University, Portland, OR, USA.
Environmental Futures Research Institute, Nathan Campus, Griffith University, Brisbane, Australia; School of Natural Sciences, Nathan Campus, Griffith University, Brisbane, Australia.
Food Microbiol. 2019 Apr;78:114-122. doi: 10.1016/j.fm.2018.10.006. Epub 2018 Oct 18.
Shewanella spp. are major causes of fish spoilage. Terminalia ferdinandiana (Kakadu plum) extracts were investigated for their ability to inhibit Shewanella spp. growth. Leaf and fruit extracts displayed potent growth inhibitory properties against all Shewanella spp. The methanolic leaf extract was a particularly potent inhibitor of S. putrefaciens (DD MIC 93; LD MIC 73 μg/mL), S. baltica (DD MIC 104 μg/mL; LD MIC 85 μg/mL), S. frigidimarina (DD MIC 466 μg/mL; LD MIC 391 μg/mL) and S. loihica (DD MIC 95 μg/mL; LD MIC 55 μg/mL) growth. The aqueous and ethyl acetate leaf extracts were also potent growth inhibitors, with MIC values generally substantially <1000 μg/mL. Treatment of Acanthopagrus butcheri Munro fillets with methanolic Kakadu plum extracts significantly inhibited bacterial growth for 15 days at 4 °C. All Kakadu plum extracts were nontoxic in the Artemia franciscana bioassay. LC-MS analysis identified several compounds which may contribute to the inhibition of Shewanella spp. growth.
希瓦氏菌属是鱼类腐败的主要原因。研究了Terminalia ferdinandiana（卡卡杜李）提取物抑制希瓦氏菌属生长的能力。叶和果实提取物对所有希瓦氏菌属均显示出强大的生长抑制特性。甲醇叶提取物对 S. putrefaciens（DD MIC 93；LD MIC 73μg/mL）、S. baltica（DD MIC 104μg/mL；LD MIC 85μg/mL）、S. frigidimarina（DD MIC 466μg/mL；LD MIC 391μg/mL）和 S. loihica（DD MIC 95μg/mL；LD MIC 55μg/mL）的生长具有特别强的抑制作用。水提和乙酸乙酯叶提取物也是强大的生长抑制剂，MIC 值通常 <1000μg/mL。用甲醇卡卡杜李提取物处理 Acanthopagrus butcheri Munro 鱼片可显著抑制细菌在 4°C 下生长 15 天。在卤虫生物测定中，所有卡卡杜李提取物均无毒性。LC-MS 分析鉴定了几种可能有助于抑制希瓦氏菌属生长的化合物。
