Microbiology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474002, India.
Microbiology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior 474002, India.
Vaccine. 2019 Jan 3;37(1):19-24. doi: 10.1016/j.vaccine.2018.11.057. Epub 2018 Nov 26.
In the current antibiotic resistance scenario, vaccines may provide best defense against lethal bacterial diseases. So far, there is no idealvaccine available against plague. Despite providing complete protection in small animal models, F1/LcrV based vaccine failed to provide ideal protection in non human primates. Here, we cloned, expressed and purified YopE of Yersinia pestis and flagellin C (FliC) of Salmonella Typhi. However the best possible protection needs the significant induction of IFN-γ and TNF-α. To determine the protective potential of the recombinant YopE alone or in formulation with FliC, Balb/C mice were immunized subcutaneously. The formulations were prepared with alum, a human compatible adjuvant. In our studies, the combination of YopE + FliC induced significantly strong humoral and cellular immune responses. A combination of YopE + FliC provided 83% protection whereas YopE alone provided only 50% against 100LD of Y. pestis in a mouse model.
在当前抗生素耐药性的情况下，疫苗可能是对抗致命细菌性疾病的最佳防御手段。目前，针对鼠疫还没有理想的疫苗。尽管在小动物模型中提供了完全的保护，但基于 F1/LcrV 的疫苗未能在非人类灵长类动物中提供理想的保护。在这里，我们克隆、表达和纯化了鼠疫耶尔森氏菌的 YopE 和伤寒沙门氏菌的鞭毛蛋白 C (FliC)。然而，要获得最佳的保护效果，需要显著诱导 IFN-γ 和 TNF-α。为了确定重组 YopE 单独或与 FliC 联合的保护潜力，Balb/C 小鼠通过皮下免疫进行了免疫。这些配方是用明矾（一种与人相容的佐剂）制备的。在我们的研究中，YopE+FliC 的联合使用显著诱导了强烈的体液和细胞免疫反应。YopE+FliC 的联合使用提供了 83%的保护，而 YopE 单独使用仅提供了 50%的保护，在小鼠模型中抵抗 100LD 的鼠疫耶尔森氏菌。
