Waldrop Steven G, Smith Garrett P, Boyle Stephen M, Sriranganathan Nammalwar
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 205 Duck Pond Dr., Blacksburg, VA 24061, USA.
Heliyon. 2021 Feb 2;7(2):e06149. doi: 10.1016/j.heliyon.2021.e06149. eCollection 2021 Feb.
Population and health management of wildlife is a key to environmental health, domestic herd health, and ultimately public health. Many different methods including: surgical sterilization, poison baits, and sponsored hunting programs have been used in the attempt to control populations of various nuisance animal species. Particular interest has been given to immunocontraception through wildlife vaccination protocols. This study specifically looked at the potential immunocontraceptive and protective properties of a RB51 Δ vaccine expressing FliC conjugated to porcine follicle stimulating hormone beta subunit (FSHβ) or gonadotropin releasing hormone (GnRH) DNA sequences. RB51 Δ pNS4--- (RB51LFSHβ) and RB51 Δ pNS4--- (RB51LGnRH) were tested in a pilot breeding study with BALB/c mice, and a significant reduction in fertility characteristics was observed in both male and female mice. Ultimately, this study provides support to test these vaccine candidates in feral swine, a destructive invasive species in the United States of America.
野生动物的种群与健康管理是环境健康、家畜健康乃至公共健康的关键。人们尝试了许多不同方法来控制各种有害动物物种的数量，包括手术绝育、毒饵以及赞助狩猎计划等。通过野生动物疫苗接种方案进行免疫避孕受到了特别关注。本研究具体考察了一种表达与猪促卵泡激素β亚基（FSHβ）或促性腺激素释放激素（GnRH）DNA序列偶联的鞭毛蛋白（FliC）的RB51Δ疫苗的潜在免疫避孕和保护特性。RB51ΔpNS4---（RB51LFSHβ）和RB51ΔpNS4---（RB51LGnRH）在一项用BALB/c小鼠进行的初步繁殖研究中进行了测试，在雄性和雌性小鼠中均观察到生育特征显著降低。最终，本研究为在野猪（美国一种具有破坏性的入侵物种）中测试这些候选疫苗提供了支持。
