Basoli Francesco, Giannitelli Sara Maria, Gori Manuele, Mozetic Pamela, Bonfanti Alessandra, Trombetta Marcella, Rainer Alberto
Department of Engineering, Università Campus Bio-Medico di Roma, Rome, Italy.
Center for Translational Medicine, International Clinical Research Center, St. Anne's University Hospital, Brno, Czechia.
Front Physiol. 2018 Nov 15;9:1449. doi: 10.3389/fphys.2018.01449. eCollection 2018.
The rapidly growing field of mechanobiology demands for robust and reproducible characterization of cell mechanical properties. Recent achievements in understanding the mechanical regulation of cell fate largely rely on technological platforms capable of probing the mechanical response of living cells and their physico-chemical interaction with the microenvironment. Besides the established family of atomic force microscopy (AFM) based methods, other approaches include optical, magnetic, and acoustic tweezers, as well as sensing substrates that take advantage of biomaterials chemistry and microfabrication techniques. In this review, we introduce the available methods with an emphasis on the most recent advances, and we discuss the challenges associated with their implementation.
力学生物学这一迅速发展的领域需要对细胞力学特性进行可靠且可重复的表征。在理解细胞命运的力学调控方面，近期的成果很大程度上依赖于能够探测活细胞力学响应及其与微环境物理化学相互作用的技术平台。除了已有的基于原子力显微镜（AFM）的方法家族外，其他方法还包括光镊、磁镊和声镊，以及利用生物材料化学和微纳加工技术的传感基底。在本综述中，我们介绍现有的方法，重点关注最新进展，并讨论与它们的实施相关的挑战。
