School of Electrical and Computer Engineering, Cornell University, Ithaca, NY, USA.
Centre de Recherche du CHU de Québec, Université Laval, Québec, Qc, Canada.
Adv Exp Med Biol. 2018;1092:319-349. doi: 10.1007/978-3-319-95294-9_15.
The forces exerted by cells on their surroundings play an integral role in both physiological processes and disease progression. Traction force microscopy is a noninvasive technique that enables the in vitro imaging and quantification of cell forces. Utilizing expertise from a variety of disciplines, recent developments in traction force microscopy are enhancing the study of cell forces in physiologically relevant model systems, and hold promise for further advancing knowledge in mechanobiology. In this chapter, we discuss the methods, capabilities, and limitations of modern approaches for traction force microscopy, and highlight ongoing efforts and challenges underlying future innovations.
细胞对周围环境施加的力在生理过程和疾病进展中都起着重要作用。牵引力显微镜是一种非侵入性技术，可实现细胞力的体外成像和定量。利用来自多个学科的专业知识，牵引力显微镜的最新进展正在增强对生理相关模型系统中细胞力的研究，并有望进一步推进机械生物学的知识。在本章中，我们讨论了现代牵引力显微镜方法的方法、功能和局限性，并强调了未来创新的现有努力和挑战。
