Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Thailand.
Integrative Complementary and Alternative Medicine Research and Development Center, Khon Kaen University, 40002, Thailand.
Biomed Res Int. 2018 Oct 25;2018:6452965. doi: 10.1155/2018/6452965. eCollection 2018.
In this study, we aimed to assess the effect and possible underlying mechanism of leaves extract on male sexual behaviors in stress-exposed rats. Male Wistar rats were orally given extract at doses of 25, 100, and 200 mg/kg BW before 12-hour-immobilization exposure for 14 days. Sexual behaviors, serum testosterone and corticosterone levels, TH-positive cells density in nucleus accumbens (NAc) and ventral tegmental area (VTA), MAO-B activity in NAc and medial preoptic area (MPOA), testis histology together with phosphodiesterase type-5 ( PDE-5) activity, and endothelial nitric oxide synthase (eNOS) expression in penis were evaluated after treatment. All doses of extract improved male sexual behaviors, suppressed MAO-B in NAc, enhanced TH-positive cells density in NAc, suppressed PDE-5 in penis, and enhanced interstitial cell of Leydig. The increase of serum testosterone, TH-positive cells density in VTA, eNOS expression in penis, and the decreased serum corticosterone were observed at some doses. Therefore, the sexual enhancing effect of extract occurred mainly via the improved dopaminergic and testicular functions. PDE-5 suppression in penis also played the role especially in the increased intromission behavior. Therefore, leaves extract is the potential protective agent against sexual dysfunction. However, further researches are necessary.
在这项研究中，我们旨在评估 叶提取物对应激暴露大鼠雄性性行为的影响及其可能的潜在机制。雄性 Wistar 大鼠在 12 小时束缚暴露前 14 天每天口服给予 叶提取物 25、100 和 200mg/kg BW。治疗后评估性行为、血清睾酮和皮质酮水平、伏隔核（NAc）和腹侧被盖区（VTA）中 TH 阳性细胞密度、NAc 和内侧视前区（MPOA）中的 MAO-B 活性、睾丸组织学以及阴茎中的磷酸二酯酶 5（PDE-5）活性和内皮型一氧化氮合酶（eNOS）表达。提取物的所有剂量均改善了雄性性行为，抑制了 NAc 中的 MAO-B，增加了 NAc 中的 TH 阳性细胞密度，抑制了阴茎中的 PDE-5，并增强了间质细胞的莱迪希。在某些剂量下观察到血清睾酮、VTA 中 TH 阳性细胞密度、阴茎中 eNOS 表达的增加和血清皮质酮的减少。因此，提取物的增强性行为作用主要是通过改善多巴胺能和睾丸功能发生的。阴茎中 PDE-5 的抑制也发挥了作用，特别是在增加插入行为方面。因此， 叶提取物是一种潜在的对抗性功能障碍的保护剂。然而，还需要进一步的研究。
