Sharon Galit, Ucko Michal, Tamir Ben, Diamant Arik
Israel Oceanographic and Limnological Research, National Center for Mariculture, P.O.Box 1212, 88112, Eilat, Israel.
Parasitol Res. 2019 Jan;118(1):159-167. doi: 10.1007/s00436-018-6151-9. Epub 2018 Nov 29.
Gray mullet (Mugilidae) occur in all seas and are farmed widely around the world, and thus, the risk of their parasites spreading through transport of aquaculture seed is a serious concern. Among others, gray mullets typically host a diversity of myxosporeans, a group in which spore morphometrics of genera has been consistently shown to be inadequate for determination of species. In this study, we investigated Myxobolus Bütschli 1882 (Myxosporea) species found in two fingerling stocks of Mugil cephalus caught in the wild off the coasts of the eastern (Israel) and western (Spain) Mediterranean. Although we observed similar morphological features, significant dissimilarities in spore size and differences in Myxobolus species SSU rDNA sequences were noted. Genetic analyses demonstrated that multiple Myxobolus species, some with SSU rDNA sequences new to GenBank, infected the stock from Spain. In addition, Myxobolus DNA was found associated with several types of host tissue (gill, tail, and internal organs), and sequence analyses indicated that multiple species of Myxobolus were also present, sometimes in different tissues from the same fish. The results suggest that the gray mullets supported a collection of several different Myxobolus species with similar morphology.
鲻鱼（鲻科）分布于所有海域，在世界各地广泛养殖，因此，其寄生虫通过水产养殖苗种运输传播的风险备受关注。其中，鲻鱼通常寄生多种粘孢子虫，一直以来，该类群属的孢子形态测量法被证明不足以确定物种。在本研究中，我们调查了在东地中海（以色列）和西地中海（西班牙）沿海捕获的野生鲻鱼苗种中发现的粘体虫属（Myxobolus Bütschli，1882年）（粘孢子虫纲）物种。尽管我们观察到相似的形态特征，但孢子大小存在显著差异，并且粘体虫属物种的小亚基核糖体DNA序列也有所不同。遗传分析表明，多种粘体虫属物种感染了来自西班牙的鱼苗种，其中一些物种的小亚基核糖体DNA序列对GenBank来说是新的。此外，在几种类型的宿主组织（鳃、尾和内脏器官）中发现了粘体虫属的DNA，序列分析表明也存在多种粘体虫属物种，有时在同一条鱼的不同组织中。结果表明，鲻鱼体内存在几种形态相似的不同粘体虫属物种。
