1Sorbonne University, Université de Technologie de Compiègne, CNRS, UMR 7338 Biomechanics and Bioengineering, Centre de Recherche Royallieu, Compiègne Cedex, France.
2Department of Dermatology, CHU Amiens Picardie-Site Nord, Amiens, France.
Tissue Eng Part A. 2019 Aug;25(15-16):1116-1126. doi: 10.1089/ten.TEA.2018.0210. Epub 2019 Jan 18.
Three dimensional cell culture systems better reflect the native structural architecture of tissues and are attractive to investigate cancer cell sensitivity to drugs. We have developed and compared several metastatic melanoma (MM) models cultured as a monolayer (2D) and cocultured on three dimensional (3D) dermal equivalents with fibroblasts to better unravel factors modulating cell sensitivity to vemurafenib, a BRAF inhibitor. The heterotypic 3D melanoma model we have established summarizes paracrine signalization by stromal cells and type I collagen matrix, mimicking the natural microenvironment of cutaneous MM, and allows for the identification of potent sensitive melanoma cells to the drug. This model could be a powerful tool for predicting drug efficiency.
三维细胞培养系统更好地反映了组织的固有结构架构，并且对于研究癌细胞对药物的敏感性很有吸引力。我们已经开发并比较了几种作为单层（2D）培养的转移性黑色素瘤（MM）模型，以及与成纤维细胞共培养的三维（3D）真皮等效物，以更好地揭示调节细胞对vemurafenib（一种 BRAF 抑制剂）敏感性的因素。我们建立的异质 3D 黑色素瘤模型概括了基质细胞和 I 型胶原基质的旁分泌信号转导，模拟了皮肤 MM 的天然微环境，并允许鉴定对药物敏感的黑色素瘤细胞。该模型可能是预测药物疗效的有力工具。
