Department of Reproduction and Genetics, the First Affiliated Hospital of Kunming Medical University, Kunming 650032, China; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, No.13 Hang Kong Road, Wuhan Hubei 430030, China.
Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, No.13 Hang Kong Road, Wuhan Hubei 430030, China.
Reprod Biomed Online. 2019 Jan;38(1):108-117. doi: 10.1016/j.rbmo.2018.11.002. Epub 2018 Nov 23.
Is humanin present in the human ovary and follicular fluid? What relationship exists between humanin concentration in the follicular fluid and ovarian reserve and clinical outcomes after IVF and intracytoplasmic sperm injection (ICSI)?
Follicular fluid samples were collected from 179 patients undergoing their first IVF or ICSI cycle during oocyte retrieval. Ovarian tissues were collected from two patients undergoing surgery for ovarian cysts. Ovarian humanin localization was analysed using immunofluorescence staining. Expression of humanin in granulosa cells was confirmed by reverse transcription polymerase chain reaction (RT-PCR) analysis. Follicular fluid humanin levels were evaluated with enzyme-linked immunosorbent assay. Relationships between follicular fluid humanin levels and ovarian reserve markers and clinical outcomes were analysed.
Strong humanin expression was found in the granulosa cells, oocytes and stromal cells of the ovary. Agarose gel electrophoresis of RT-PCR products showed rich humanin mRNA expression in human granulosa cells (119 bp). Follicular fluid humanin concentrations ranged from 86.40 to 417.60 pg/ml. They significantly correlated with FSH (r = -0.21; P < 0.01), LH (r = -0.18; P = 0.02), antral follicle count (r = 0.27; P < 0.01), anti-Müllerian hormone (r = 0.24; P = 0.03) and inhibin B (r = 0.46; P < 0.01) levels. Patients were subdivided into four groups according to follicular fluid humanin concentration quartiles (Q1-Q4). Patients in Q4 were more likely to achieve a pregnancy than Q1 (OR = 3.60; 95% CI 1.09 to 11.84).
Humanin concentration in the follicular fluid was positively associated with ovarian reserve and clinical pregnancy rate.
人类素是否存在于人类卵巢和卵泡液中？卵泡液中人类素的浓度与卵巢储备以及体外受精和胞浆内单精子注射（ICSI）后的临床结局之间存在什么关系？
从 179 名接受取卵的患者的卵泡液中采集样本。从两名因卵巢囊肿手术的患者中采集卵巢组织。使用免疫荧光染色分析卵巢中人素的定位。通过逆转录聚合酶链反应（RT-PCR）分析确认颗粒细胞中人素的表达。用酶联免疫吸附试验评估卵泡液中人素的水平。分析卵泡液中人素水平与卵巢储备标志物和临床结局之间的关系。
在卵巢的颗粒细胞、卵子和基质细胞中发现了强烈的人类素表达。RT-PCR 产物的琼脂糖凝胶电泳显示人颗粒细胞中人类素 mRNA 表达丰富（119bp）。卵泡液中人素浓度范围为 86.40 至 417.60pg/ml。它们与 FSH（r= -0.21；P < 0.01）、LH（r= -0.18；P= 0.02）、窦卵泡计数（r= 0.27；P < 0.01）、抗苗勒管激素（r= 0.24；P= 0.03）和抑制素 B（r= 0.46；P < 0.01）水平呈显著正相关。根据卵泡液中人素浓度四分位（Q1-Q4）将患者分为四组。与 Q1 相比，Q4 患者更有可能实现妊娠（OR=3.60；95%CI 1.09 至 11.84）。
卵泡液中人类素的浓度与卵巢储备和临床妊娠率呈正相关。
