Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
Stem Cell Reports. 2018 Dec 11;11(6):1347-1356. doi: 10.1016/j.stemcr.2018.10.023. Epub 2018 Nov 29.
Nicotinamide, the amide form of vitamin B3, is widely used in disease treatments and stem cell applications. However, nicotinamide's impact often cannot be attributed to its nutritional functions. In a vitamin screen, we find that nicotinamide promotes cell survival and differentiation in human pluripotent stem cells. Nicotinamide inhibits the phosphorylation of myosin light chain, suppresses actomyosin contraction, and leads to improved cell survival after individualization. Further analysis demonstrates that nicotinamide is an inhibitor of multiple kinases, including ROCK and casein kinase 1. We demonstrate that nicotinamide affects human embryonic stem cell pluripotency and differentiation as a selective kinase inhibitor. The findings in this report may help researchers design better strategies to develop nicotinamide-related stem cell applications and disease treatments.
烟酰胺，维生素 B3 的酰胺形式，被广泛应用于疾病治疗和干细胞应用中。然而，烟酰胺的作用往往不能归因于其营养功能。在维生素筛选中，我们发现烟酰胺可促进人多能干细胞的存活和分化。烟酰胺抑制肌球蛋白轻链的磷酸化，抑制肌动球蛋白收缩，从而提高细胞在个体化后的存活率。进一步的分析表明，烟酰胺是多种激酶的抑制剂，包括 ROCK 和酪蛋白激酶 1。我们证明烟酰胺作为一种选择性激酶抑制剂影响人类胚胎干细胞的多能性和分化。本报告中的发现可能有助于研究人员设计更好的策略来开发烟酰胺相关的干细胞应用和疾病治疗。
