工程化干细胞类器官
Engineering Stem Cell Organoids.
作者信息
Yin Xiaolei, Mead Benjamin E, Safaee Helia, Langer Robert, Karp Jeffrey M, Levy Oren
机构信息
Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Cambridge, MA 02115, USA; Harvard Medical School, Cambridge, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Harvard - MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
出版信息
Cell Stem Cell. 2016 Jan 7;18(1):25-38. doi: 10.1016/j.stem.2015.12.005.
Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.
类器官系统利用干细胞的自我组织特性来创建各种多细胞组织替代物。大多数类器官模型仅代表组织的单个或部分组成部分，并且在这些系统中通常难以控制细胞类型、组织以及细胞-细胞/细胞-基质相互作用。在此，我们讨论生成基于干细胞的类器官的基本方法、它们的优点和局限性，以及如何利用生物工程策略来调控类器官内的细胞组成及其三维组织，以进一步提高它们在研究和治疗中的效用。
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