Inserm U1065, C3M, Team Control of Gene Expression (10), Nice, France; Université Côte d'Azur, Inserm, C3M, Nice, France.
Inserm U1065, C3M, Team Control of Gene Expression (10), Nice, France; Université Côte d'Azur, Inserm, C3M, Nice, France.
Trends Genet. 2019 Jan;35(1):15-28. doi: 10.1016/j.tig.2018.10.006. Epub 2018 Nov 30.
Different methods have recently been developed to understand the subcellular localization and role of microRNAs (miRNAs) as well as small RNAs associated with Argonaute (AGO) proteins. The heterogeneity of the protein complexes associated with miRNAs, along with their subcellular localization, provides clues into their biochemical mechanism of function. Subcellular diversity indicates that miRNAs localized to different cellular regions could have different functions, including transcriptional regulation on chromatin or post-transcriptional control, providing global regulation of gene expression by miRNAs. Herein, I review the current knowledge and most recent discoveries relating to the subcellular function of miRNAs and other AGO-associated small RNAs, revealing the emergence of a multitude of functions of the miRNA pathway to control different steps of the gene expression program(s).
最近已经开发出不同的方法来了解 microRNAs（miRNAs）以及与 Argonaute（AGO）蛋白相关的小 RNA 的亚细胞定位和作用。与 miRNAs 相关的蛋白质复合物的异质性及其亚细胞定位为其生化功能机制提供了线索。亚细胞多样性表明定位于不同细胞区域的 miRNAs 可能具有不同的功能，包括染色质上的转录调控或转录后控制，通过 miRNAs 对基因表达进行全局调控。在此，我综述了与 miRNAs 和其他 AGO 相关小 RNA 的亚细胞功能相关的最新知识和最新发现，揭示了 miRNA 通路的多种功能的出现，以控制基因表达程序的不同步骤。
