miRNA 靶向：超越种子。
miRNA Targeting: Growing beyond the Seed.
机构信息
Division of Biology, University of California-San Diego, La Jolla, CA 92093-0349, USA.
Division of Biology, University of California-San Diego, La Jolla, CA 92093-0349, USA.
出版信息
Trends Genet. 2019 Mar;35(3):215-222. doi: 10.1016/j.tig.2018.12.005. Epub 2019 Jan 9.
Abstract
miRNAs are small RNAs that guide Argonaute proteins to specific target mRNAs to repress their translation and stability. Canonically, miRNA targeting is reliant on base pairing of the seed region, nucleotides 2-7, of the miRNA to sites in mRNA 3' untranslated regions. Recently, the 3' half of the miRNA has gained attention for newly appreciated roles in regulating target specificity and regulation. In addition, the extent of pairing to the miRNA 3' end can influence the stability of the miRNA itself. These findings highlight the importance of sequences beyond the seed in controlling the function and existence of miRNAs.
摘要
miRNAs 是一种小 RNA，它可以引导 Argonaute 蛋白与特定的靶 mRNA 结合，从而抑制其翻译和稳定性。通常情况下，miRNA 的靶向作用依赖于 miRNA 种子区域（核苷酸 2-7）与 mRNA 3'非翻译区（UTR）位点的碱基配对。最近，miRNA 的 3' 端的作用也引起了人们的关注，因为人们新发现它在调节靶标特异性和调控方面具有作用。此外，miRNA 3' 端的配对程度也会影响 miRNA 本身的稳定性。这些发现强调了种子区域以外的序列在控制 miRNA 的功能和存在方面的重要性。
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2
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3
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7
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8
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