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非洲柏木的抗氧化和促氧化作用可能是其在非洲传统医学中广泛应用的基础。

Antioxidant and prooxidant effects of Piptadeniastrum africanum as the possible rationale behind its broad scale application in African ethnomedicine.

机构信息

Department of Applied Chemistry, Faculty of Science, University of Johannesburg, PO Box 17011, Doornfontein 2028, Johannesburg, South Africa.

Department Chemistry and Biochemistry, Medical Faculty, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria.

出版信息

J Ethnopharmacol. 2019 Mar 1;231:429-437. doi: 10.1016/j.jep.2018.11.039. Epub 2018 Nov 30.

DOI:10.1016/j.jep.2018.11.039

PMID:30503766

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Piptadeniastrum africanum is widely used in treating oxidative stress related diseases. Oxidative stress, defined as the disturbance in the balance between the production of free radicals and antioxidant defenses, is the root cause of many pathophysiological conditions. Based on the dual properties of prooxidants as toxic and beneficial compounds, both prooxidants and antioxidants may be effective in the treatment of these conditions when the right dose is given to the right subject at the right time for the right duration.

AIM OF THE STUDY

This study was aimed at investigating the in vitro and ex vivo anti- and pro-oxidative effects of P. africanum.

MATERIALS AND METHODS

Total phenolic and flavonoid contents of methanol and aqueous extracts of P. africanum stem back were quantified spectrophotometrically. The methanol extract, ascorbate radicals and reactive oxygen species in brain and liver homogenates of mice treated with the methanol stem bark extract were analyzed by electron paramagnetic resonance (EPR) spectroscopy. Free radical scavenging of DPPH was determined by spectrophotometric and EPR assays.

RESULTS

The methanol extract was richer in both phenolic and flavonoid contents compared to the aqueous extracts and also showed better DPPH radical scavenging capacity. The EPR spectroscopy in vitro analysis exhibited high DPPH scavenging capacity before and after UV irradiation (99.5% and 98.76%) at 40 μg/ml extract. The ex vivo EPR spectroscopy studies demonstrated increased levels of ascorbate radicals (•Asc) in liver and brain homogenates of healthy mice treated with P. africanum in comparison with those of the non treated controls (0.6141 ± 0.026 vs 0.1800 ± 0.0073 arb. units for liver homogenates and 0.9605 ± 0.0492 vs 0.3375 ± 0.0062 arb. units for brain homogenates, correspondingly). Considerably, higher levels of reactive oxygen species (ROS) were measured in mice liver and brain homogenates after treatment with P. africanum extract compared to the control group, as well (1.9402 ± 0.1200 vs 0.6699 ± 0.062 arb. units for liver homogenates and 1.7325 ± 01503 vs 0.3167 ± 0.0403 arb.units, respectively).

CONCLUSION

Therefore, P. africanum exhibited antioxidant and pro-oxidant properties which may explain its broad spectrum use in a wide variety of ailments.

摘要

民族药理学相关性

非洲白檀被广泛用于治疗与氧化应激相关的疾病。氧化应激是指自由基产生与抗氧化防御之间的平衡被打破，是许多病理生理状况的根本原因。基于促氧化剂既是有毒化合物又是有益化合物的双重特性，在给予正确剂量、正确时间和正确持续时间的情况下，促氧化剂和抗氧化剂都可能对这些情况的治疗有效。

研究目的

本研究旨在研究非洲白檀的体外和体内抗-和促氧化作用。

材料和方法

使用分光光度法定量测定非洲白檀茎背面甲醇和水提取物中的总酚和类黄酮含量。通过电子顺磁共振（EPR）光谱分析用甲醇树皮提取物处理的小鼠脑和肝匀浆中的抗坏血酸自由基和活性氧物质。通过分光光度法和 EPR 测定法测定 DPPH 的自由基清除能力。

结果

与水提取物相比，甲醇提取物中的酚类和类黄酮含量都更丰富，并且对 DPPH 自由基的清除能力也更好。体外 EPR 光谱分析表明，在 40μg/ml 提取物时，提取物在紫外线照射前后均具有高的 DPPH 清除能力（99.5%和 98.76%）。离体 EPR 光谱研究表明，与未处理的对照组相比，用非洲白檀处理的健康小鼠的肝和脑匀浆中的抗坏血酸自由基（•Asc）水平升高（肝匀浆分别为 0.6141±0.026 和 0.1800±0.0073 arb.单位，脑匀浆分别为 0.9605±0.0492 和 0.3375±0.0062 arb.单位）。相当大的是，与对照组相比，用非洲白檀提取物处理的小鼠肝和脑匀浆中的活性氧物质（ROS）水平也更高（肝匀浆分别为 1.9402±0.1200 和 0.6699±0.062 arb.单位，脑匀浆分别为 1.7325±01503 和 0.3167±0.0403 arb.units）。