Ateufack Gilbert, Domgnim Mokam Elisabeth Carol, Mbiantcha Marius, Dongmo Feudjio Rostand Breuil, David Nana, Kamanyi Albert
Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Department of Animal Biology, Dschang University, Animal Physiology and Phytopharmacology Laboratory, P.O. Box 67, Dschang, Cameroon.
BMC Complement Altern Med. 2015 Jul 8;15:214. doi: 10.1186/s12906-015-0713-5.
Gastric peptic ulcer is one of the common disorders of gastrointestinal tract, which occur due to an imbalance between the offensive and defensive factors. It is an illness that affects a considerable number of people worldwide. This study was conducted to evaluate the antiulcerogenic and antiulcer effects and recognize the basic mechanism of action of Piptadeniastrum africanum stem bark extracts.
The aqueous and methanol extracts of Piptadeniastrum africanum were administered at the doses 125, 250 and 500 mg/kg to evaluate their effects on gastric ulcer induced by the HCl/ethanol mixture, indomethacin and acetic acid in Wistar strain male adult rats, aged between 12 and 16 weeks and weighing between 180 and 220 g. Ranitidine, Maalox and Misoprostol were used as standard drugs. Histopathological examination and nitric oxide level were performed to evaluate the basic mechanism of action of Piptadeniastrum africanum. Phytochemical screening was carried out to identify known phytochemicals present in these extracts.
The aqueous and methanol extracts of stem bark of Piptadeniastrum africanum significantly inhibited (p < 0.01) gastric ulceration induced by HCl/ethanol to the percentages of inhibition of 81.38; 98.75 and 100 % for the aqueous extract and then 75.83, 89.76 and 96.52 % for the methanol extract, and with the Indomethacin-induced ulcers, aqueous and methanol extracts of bark of Piptadeniastrum africanum reduce significantly (p < 0.01) induced gastric lesions in rats, with percentage of cure 35.75; 52.33 and 98.58 % for the aqueous extract, and 33.7; 51.97; and 65.93 to the methanol extract. The results revealed a significant reduction of ulcerated surface in both extracts and increase of nitric oxide (NO) level with methanol extract. When compared to methanol extract, aqueous extract showed more pronounced effects, corresponding to percentages of healing of 59. 92; 84.12 and 59.65 % for the aqueous extract; and 70.43; 55.49 and 57.59 % for the methanol extract in the ulcer induced by acetic acid, all at the respective doses of 125, 250 and 500 mg/kg. Histopathological observations also demonstrated curative effect. As such, both extracts were found to exhibit preventive and curative effects through the release of NO and growth factors. This could also be due to the presence of phytochemicals such as alkaloids, flavonoids, phenols and saponins which act as antisecretory agents.
Piptadeniastrum africanum stem bark extracts thus have gastroprotective and ulcer healing effects, which could result from their activities by stimulating important cellular mechanisms such as migration and proliferation of epithelial cells that may have a cytoprotective effect by stimulating the release of prostaglandins. These results are required to confirm the ethnopharmacological use of Piptadeniastrum africanum stem bark in the treatment of ulcer.
胃溃疡是常见的胃肠道疾病之一，其发生是由于攻击因素和防御因素之间的失衡。这是一种影响全球相当多人群的疾病。本研究旨在评估非洲风车子茎皮提取物的抗溃疡形成和抗溃疡作用，并认识其基本作用机制。
将非洲风车子的水提取物和甲醇提取物分别以125、250和500mg/kg的剂量给药，以评估它们对12至16周龄、体重180至220g的Wistar品系雄性成年大鼠中由盐酸/乙醇混合物、吲哚美辛和乙酸诱导的胃溃疡的影响。雷尼替丁、氢氧化铝镁和米索前列醇用作标准药物。进行组织病理学检查和一氧化氮水平检测以评估非洲风车子的基本作用机制。进行植物化学筛选以鉴定这些提取物中存在的已知植物化学物质。
非洲风车子茎皮的水提取物和甲醇提取物均显著抑制（p＜0.01）由盐酸/乙醇诱导的胃溃疡，水提取物的抑制率分别为81.38%、98.75%和100%，甲醇提取物的抑制率分别为75.83%、89.76%和96.52%；对于吲哚美辛诱导的溃疡，非洲风车子茎皮的水提取物和甲醇提取物均显著（p＜0.01）减轻大鼠胃损伤，水提取物的治愈率分别为35.75%、52.33%和98.58%，甲醇提取物的治愈率分别为33.7%、51.97%和65.93%。结果显示两种提取物均显著减少溃疡表面，且甲醇提取物使一氧化氮（NO）水平升高。与甲醇提取物相比，水提取物显示出更显著的效果，在乙酸诱导的溃疡中，水提取物在125、250和500mg/kg各自剂量下的愈合率分别为59.92%、84.12%和59.65%，甲醇提取物的愈合率分别为70.43%、55.49%和57.59%。组织病理学观察也证实了治疗效果。因此，发现两种提取物均通过释放NO和生长因子发挥预防和治疗作用。这也可能是由于存在生物碱、黄酮类、酚类和皂苷等植物化学物质，它们作为抗分泌剂发挥作用。
非洲风车子茎皮提取物具有胃保护和溃疡愈合作用，这可能是由于它们通过刺激重要的细胞机制（如上皮细胞的迁移和增殖）发挥作用，这些机制可能通过刺激前列腺素的释放而具有细胞保护作用。这些结果需要证实非洲风车子茎皮在治疗溃疡方面的民族药理学用途。
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