Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal 713 104, India.
Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, West Bengal 713 104, India.
Int J Biol Macromol. 2019 Mar 15;125:580-587. doi: 10.1016/j.ijbiomac.2018.11.117. Epub 2018 Nov 30.
Thymus vulgaris is used for various health benefits and culinary, nevertheless, report on its macromolecules is absent. Here, we report chemical compositions of the polysaccharides isolated from its leaf by sequential extraction with inorganic solvents. In particular, chemical profile of a unique rhamnogalacturonan I type polysaccharide containing ester linked phenolic acids has been described. Sugar compositional, TLC, UV-spectrometric and ESI-MS analyses of oligosaccharides generated from this polysaccharide by enzyme digestion, controlled acid hydrolysis and Smith degradation revealed atypical fine structural details. Biochemical analysis demonstrated dose-dependent antioxidant activity. A combination of large neutral side chains of the ramified region and ester linked phenolic acids are regarded as the functional sites. Ultraviolet spectrometric and fluorimetric analyses showed that this polysaccharide forms a homogeneous water-soluble complex with bovine serum albumin (binding constant, K = 2.91 × 10/M). Consequently, water extraction affords a polysaccharide which induces pharmacological effect; this underlines the impact of thyme as natural dietetic antioxidant.
普通百里香被用于各种健康益处和烹饪，然而，关于其大分子的报告却很少。在这里，我们报告了从其叶子中用无机溶剂顺序提取分离得到的多糖的化学成分。特别是，描述了一种含有酯键连接的酚酸的独特鼠李半乳糖醛酸 I 型多糖的化学特征。通过酶解、控制酸水解和 Smith 降解从这种多糖生成的低聚糖的糖组成、TLC、紫外光谱和 ESI-MS 分析揭示了非典型的精细结构细节。生化分析表明该多糖具有剂量依赖性的抗氧化活性。支化区域的大中性侧链和酯键连接的酚酸组合被认为是功能位点。紫外光谱和荧光分析表明，这种多糖与牛血清白蛋白形成均一的水溶性复合物（结合常数，K=2.91×10/M）。因此，水提得到一种具有药理作用的多糖；这强调了百里香作为天然饮食抗氧化剂的影响。
