Ren Fei, Dong Wei, Yan Dong-Hui
Forestry Experiment Center in North China, Chinese Academy of Forestry, Beijing, 102300, China.
China Electric Power Research Institute, Beijing, 100192, China.
Arch Microbiol. 2019 Mar;201(2):199-208. doi: 10.1007/s00203-018-1597-9. Epub 2018 Dec 1.
Plant endophytes play a crucial role in plant growth, health and ecological function. Jingbai pear (the best quality cultivar of Pyrus ussuriensi Maxim. ex Rupr.) has important ecological and economic value in north China. Conversation of its genetics has great meanings to pear genus (Pyrus L.). However, the bacterial community associated with the cultivar remains unknown. In this study, the structure of endophytic bacterial communities associated with different tissues and soil of Jingbai Pear trees was analyzed with Illumina Miseq sequencing of bacterial 16S rDNA. This is the first report on bacterial microbiome associated with Jingbai pear. Our results demonstrated that different tissues harbored a unique bacterial assemblage. Interestingly, Cyanobacteria was the most abundant phylum, followed by Proteobacteria and Actinobacteria. Samples from three different sites (soils) had significant differences in microbial communities structure. Redundancy analysis (RDA) showed that the bacterial community structure correlated significantly with soil properties-temperature, pH, nitrogen and carbon contents. The conclusion could facilitate to understand the interaction and ecological function of endophytic bacteria with host Jingbai pear trees, so as to benefit the pear variety genetic resource conservation and protection.
植物内生菌在植物生长、健康和生态功能方面发挥着关键作用。京白梨（秋子梨的最优品种）在中国北方具有重要的生态和经济价值。其遗传资源的保存对梨属植物具有重大意义。然而，与该品种相关的细菌群落仍不为人所知。在本研究中，利用细菌16S rDNA的Illumina Miseq测序分析了京白梨树不同组织和土壤中内生细菌群落的结构。这是关于京白梨细菌微生物组的首次报道。我们的结果表明，不同组织中存在独特的细菌组合。有趣的是，蓝细菌是最丰富的门类，其次是变形菌门和放线菌门。来自三个不同地点（土壤）的样本在微生物群落结构上存在显著差异。冗余分析（RDA）表明细菌群落结构与土壤性质——温度、pH值、氮和碳含量显著相关。该结论有助于理解内生细菌与宿主京白梨树的相互作用及生态功能，从而有利于梨品种遗传资源的保存和保护。
