Padda Kiran Preet, Puri Akshit, Nguyen Nguyen Khoi, Philpott Timothy J, Chanway Chris P
Department of Forest and Conservation Sciences, Faculty of Forestry, The University of British Columbia, Vancouver, BC Canada.
Present Address: School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland.
Plant Soil. 2022;474(1-2):213-232. doi: 10.1007/s11104-022-05327-2. Epub 2022 Mar 9.
Despite little soil development and organic matter accumulation, lodgepole pine () consistently shows vigorous growth on bare gravel substrate of aggregate mining pits in parts of Canadian sub-boreal forests. This study aimed to investigate the bacterial microbiome of lodgepole pine trees growing at an unreclaimed gravel pit in central British Columbia and suggest their potential role in tree growth and survival following mining activity.
We characterized the diversity, taxonomic composition, and relative abundance of bacterial communities in rhizosphere and endosphere niches of pine trees regenerating at the gravel pit along with comparing them with a nearby undisturbed forested site using 16S rRNA high-throughput sequencing. Additionally, the soil and plant nutrient contents at both sites were also analyzed.
Although soil N-content at the gravel pit was drastically lower than the forest site, pine tissue N-levels at both sites were identical. Beta-diversity was affected by site and niche-type, signifying that the diversity of bacterial communities harboured by pine trees was different between both sites and among various plant-niches. Bacterial alpha-diversity was comparable at both sites but differed significantly between belowground and aboveground plant-niches. In terms of composition, pine trees predominantly associated with taxa that appear plant-beneficial including phylotypes of , , and at the gravel pit and , , and at the forest site.
Our results suggest that, following mining activity, regenerating pine trees recruit bacterial communities that could be plant-beneficial and support pine growth in an otherwise severely N-limited disturbed environment.
The online version contains supplementary material available at 10.1007/s11104-022-05327-2.
尽管土壤发育程度低且有机质积累少，但在加拿大亚寒带森林部分地区的集料矿坑裸露砾石基质上，黑松（）始终呈现出旺盛的生长态势。本研究旨在调查生长在不列颠哥伦比亚省中部一个未复垦砾石坑的黑松的细菌微生物组，并探讨其在采矿活动后树木生长和存活中的潜在作用。
我们利用16S rRNA高通量测序对在砾石坑再生的松树根际和内生生态位中的细菌群落的多样性、分类组成和相对丰度进行了表征，并将其与附近未受干扰的森林站点进行了比较。此外，还分析了两个站点的土壤和植物养分含量。
尽管砾石坑的土壤氮含量远低于森林站点，但两个站点的松树组织氮水平相同。β多样性受站点和生态位类型的影响，这表明两个站点之间以及不同植物生态位之间，松树所拥有的细菌群落多样性存在差异。两个站点的细菌α多样性相当，但地下和地上植物生态位之间存在显著差异。在组成方面，松树主要与一些对植物有益的分类群相关联，在砾石坑包括、和的系统发育型，在森林站点包括、、和。
我们的结果表明，采矿活动后，再生松树招募的细菌群落可能对植物有益，并在原本严重氮受限的干扰环境中支持松树生长。
在线版本包含可在10.1007/s11104-022-05327-2获取的补充材料。
