单纯疱疹病毒对阿昔洛韦的敏感性——基因型和表型特征
HSV susceptibility to acyclovir - genotypic and phenotypic characterization.
作者信息
Vikas Raksha, Prabhu Suresha G, Mudgal Piya P, Shetty Ujwal, Karunakaran Kavitha, Jagadesh Anitha, Auti Amogh, Stansilaus Rithu P, Nair Sudheesh, Arunkumar Govindakarnavar
机构信息
Manipal Centre for Virus Research, Manipal Academy of Higher Education (deemed to be University), Manipal, Karnataka, India.
出版信息
Antivir Ther. 2019;24(2):141-145. doi: 10.3851/IMP3279.
BACKGROUND
Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples.
METHODS
A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique.
RESULTS
Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay.
CONCLUSIONS
These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.
背景
耐药性单纯疱疹病毒（HSV）引起的感染是一个重要的临床问题，尤其是在免疫功能低下的患者中。本研究旨在检测HSV临床样本中阿昔洛韦（ACV）的敏感性。
方法
本研究纳入了从患者中获得的13份HSV阳性临床样本（5份HSV-1和8份HSV-2），这些患者中1例免疫功能低下，12例免疫状态未知。基因分型分析首先进行UL23（胸苷激酶）基因测序，然后使用蚀斑减少技术进行确证性表型分析。
结果
在HSV-1阳性临床样本中检测到两个新的氨基酸变化，A37V和H283N，通过蚀斑减少试验发现这些样本对阿昔洛韦敏感（半数最大效应浓度=1.5μM）。
结论
因此，这两个新的氨基酸变化可被视为自然多态性，这是一种与HSV-UL23基因广泛相关的现象。
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