Oliveira-Klein Mariana de, Cardoso-Dos-Santos Augusto César, Tagliani-Ribeiro Alice, Fagundes Nelson Rosa, Matte Ursula, Schuler-Faccini Lavinia
Departmento de Genética, Instituto de Bociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Instituto Nacional de Ciência e Tecnologia de Genética Médica Populacional(INaGeMP), Departmento de Genética, Instituto de Bociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Genet Mol Biol. 2018 Oct-Dec;41(4):775-780. doi: 10.1590/1678-4685-GMB-2017-0263. Epub 2018 Nov 29.
Insulin-like growth factor (IGF-1) is an important peptide hormone involved in the reproduction and fetal development of mammals, and it is suggested that it may influence the human twinning rate. This study aimed to test such possible association, investigating the genetic polymorphisms IGF1 (CA)n and IGFBP3 rs2854744 in the population from Candido Godoi (CG), a small city located in the South of Brazil that has a high prevalence of twin births. A case-control study was performed comprising a total of 39 cases (representing about 40% of the mothers of twins who were born in CG after 1995) and 214 controls (mothers of non-twin children), 97 of whom were living in CG while 117 were living in Porto Alegre. DNA was extracted from blood leucocytes and genotyping was performed. According to the statistical analyses, there was no significant difference in the frequencies of both studied genetic polymorphisms when comparing case group with control group. Thus, our results pointed to a lack of association between IGF1 (CA)n and IGFBP3 rs2854744 polymorphisms and twin births in CG, but further investigations in other populations with different characteristics must be performed to confirm the role of IGF-I in human twinning.
胰岛素样生长因子（IGF-1）是一种重要的肽类激素，参与哺乳动物的生殖和胎儿发育，有人认为它可能影响人类的双胞胎出生率。本研究旨在检验这种可能的关联，调查位于巴西南部的小城坎迪多·戈多伊（CG）人群中IGF1（CA）n和IGFBP3 rs2854744的基因多态性，该城双胞胎出生率很高。进行了一项病例对照研究，共纳入39例病例（约占1995年后在CG出生的双胞胎母亲的40%）和214例对照（非双胞胎孩子的母亲），其中97例居住在CG，117例居住在阿雷格里港。从血液白细胞中提取DNA并进行基因分型。根据统计分析，比较病例组和对照组时，所研究的两种基因多态性的频率没有显著差异。因此，我们的结果表明CG人群中IGF1（CA）n和IGFBP3 rs2854744多态性与双胞胎出生之间缺乏关联，但必须在其他具有不同特征的人群中进行进一步研究，以证实IGF-I在人类双胞胎出生中的作用。
