School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Institute of Product Quality Supervision and Inspection, Hefei 230051, China.
Int J Biol Macromol. 2019 Mar 1;124:972-980. doi: 10.1016/j.ijbiomac.2018.11.276. Epub 2018 Dec 1.
Ginkgo biloba leaves (GBLs) are used as herbal dietary supplements and medicine worldwide. In this study, crude GBL polysaccharides (GBPSs) were extracted and further purified on a DEAE (diethylaminoethanol) Sepharose Fast Flow column to obtain GBPS-2 and GBPS-3. The molecular weights of GBPS-2 and GBPS-3 were 672 and 723 kDa, respectively. GBPS-2 and GBPS-3 were typical acidic heteropolysaccharides, composed of mannose (Man), rhamnose (Rha), glucuronic acid (GlcA), galacturonic acid (GalA), glucose (Glc), galactose (Gal), and arabinose (Ara) (molar ratio: 0.08:0.12:0.16:0.06:0.11:1.00:0.32) and Man, Rha, GlcA, GalA, Gal, and Ara (molar ratio: 0.92:1.00:0.83:0.11:0.42:0.23), respectively. GBPS-2 and GBPS-3 exhibited limited scavenging abilities for the hydroxyl and 2,2‑diphenyl‑1‑picrylhydrazyl radicals as well as noticeable scavenging effects on superoxide radicals and 2,2'‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulphonic acid) radicals. Furthermore, GBPS-2 and GBPS-3 significantly increased the phagocytosis of macrophages and promoted the production of NO, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6. Thus, GBPS-2 and GBPS-3 exhibit potential application as functional food supplements.
银杏叶被广泛用作草药膳食补充剂和药物。在本研究中，从银杏叶粗多糖中提取并进一步用 DEAE（二乙氨基乙基）琼脂糖快速流动柱纯化得到 GBPS-2 和 GBPS-3。GBPS-2 和 GBPS-3 的分子量分别为 672 和 723 kDa。GBPS-2 和 GBPS-3 均为典型的酸性杂多糖，由甘露糖（Man）、鼠李糖（Rha）、葡萄糖醛酸（GlcA）、半乳糖醛酸（GalA）、葡萄糖（Glc）、半乳糖（Gal）和阿拉伯糖（Ara）（摩尔比：0.08：0.12：0.16：0.06：0.11：1.00：0.32）和 Man、Rha、GlcA、GalA、Gal 和 Ara（摩尔比：0.92：1.00：0.83：0.11：0.42：0.23）组成。GBPS-2 和 GBPS-3 对羟基和 2,2-二苯基-1-苦基肼自由基的清除能力有限，但对超氧自由基和 2,2' -联氮-双（3-乙基苯并噻唑啉-6-磺酸）自由基具有明显的清除作用。此外，GBPS-2 和 GBPS-3 显著增强了巨噬细胞的吞噬作用，促进了 NO、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6 的产生。因此，GBPS-2 和 GBPS-3 具有作为功能性食品补充剂的应用潜力。
