College of Veterinary Medicine, Yunnan Agricultural University, Kunming, 650201, China.
College of Agronomy and Life Sciences, Kunming University, Kunming, 650214, China.
BMC Vet Res. 2024 Aug 15;20(1):366. doi: 10.1186/s12917-024-04204-7.
Ovine contagious pustular dermatitis (ORF) is one of the main diseases of sheep and is a zoonotic disease caused by Ovine contagious pustular dermatitis virus (ORFV) infection, posing a significant constraint on sheep breeding industry and human health. The Tibetan medical formulation composed of Polygonum leucoides, Polygonum xanthoxylum and Acanthophora rotunda significantly regulated lymphocyte immune function following ORFV stimulation, although the mechanism remains unclear. In order to study the immunomodulatory effects and mechanism of three Tibetan medicinal extracts (Polygonum leucoides, Polygonum xanthoxylum, and Acanthophora rotunda) against ORFV in vitro, sheep peripheral blood lymphocytes were isolated in vitro and treated with different concentrations of Tibetan medicine compound extract solution after ORFV infection. The cytokine expression levels in lymphocytes were measured at 4 h, 8 h and 12 h. Additionally endogenous metabolites in lymphocytes at 0 h, 4 h, 8 h and 12 h were quantified by untargeted metabolomics method. The results showed that, the extracts could regulate the lymphocyte immune factors altered by ORFV, and regulate the lymphocyte immune function through cysteine and methionine metabolic pathways as well as the pyrimidine metabolic pathways, potentially alleviating the immune evasion induced by ORFV.
绵羊传染性脓疱性皮炎（ORF）是绵羊的主要疾病之一，是由绵羊传染性脓疱性皮炎病毒（ORFV）感染引起的一种人畜共患病，对绵羊养殖业和人类健康构成重大威胁。由白首乌、黄杨和轮叶狐尾藻组成的藏药配方在 ORFV 刺激后显著调节淋巴细胞免疫功能，但其机制尚不清楚。为了研究三种藏药提取物（白首乌、黄杨和轮叶狐尾藻）对 ORFV 的体外免疫调节作用和机制，体外分离绵羊外周血淋巴细胞，在 ORFV 感染后用不同浓度的藏药复方提取液处理。在 4h、8h 和 12h 测量淋巴细胞中细胞因子的表达水平。此外，还通过非靶向代谢组学方法定量分析 0h、4h、8h 和 12h 淋巴细胞中的内源性代谢物。结果表明，提取物可调节 ORFV 改变的淋巴细胞免疫因子，并通过半胱氨酸和蛋氨酸代谢途径以及嘧啶代谢途径调节淋巴细胞免疫功能，可能缓解 ORFV 诱导的免疫逃避。
