School of Environment and Energy, South China University of Technology, Guangzhou, 510006, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou, 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China.
Water Res. 2019 Mar 1;150:120-128. doi: 10.1016/j.watres.2018.11.040. Epub 2018 Nov 19.
Functional identification and elimination of activity-decayed sludge are helpful for improving the performance of biological treatment process. However, cell decay-associated changes in biological functions have not been explored for gravity-separated sludge. In this work, sludge flocs from the aerobic basin of a wastewater treatment plant treating high-concentration organic coking wastewater was fractionated according to settling velocity, i.e. sludge F (fast settling), sludge M (moderate settling) and sludge S (slow settling). Sludge volume index (SVI), mean floc size, dehydrogenase activity, specific oxygen uptake rate (SOUR), extracellular polymeric substances (EPS) content and aggregation interaction were investigated in the fractionated sludges. Apoptosis-like decayed cell distribution (ALDCD), a novel property of sludge, was proposed to describe sludge decay based on cell membrane variation. ALDCD of sludge F was 6.64% and 13.5% lower than sludge M and S, respectively. Microbial community and functional prediction revealed that sludge F exhibited the highest microbial potential for organic removal and sludge M had the highest potential for nitrogen metabolism while sludge S had the lowest potential for both. Our analysis suggests that the treatment efficiency might be enhanced by retaining compact sludge flocs while eliminating dispersive sludge flocs. This study also facilitates the identification and elimination of functional microbial groups from decayed sludge in wastewater treatment.
功能鉴定和活性衰减污泥的去除有助于提高生物处理工艺的性能。然而，对于重力分离的污泥，尚未探讨与细胞衰减相关的生物功能变化。在这项工作中，根据沉降速度对处理高浓度有机焦化废水的污水处理厂好氧池中的污泥絮体进行了分级，即污泥 F（快速沉降）、污泥 M（中等沉降）和污泥 S（缓慢沉降）。研究了分级污泥中的污泥体积指数（SVI）、平均絮体大小、脱氢酶活性、比需氧量（SOUR）、胞外聚合物（EPS）含量和聚集相互作用。基于细胞膜变化，提出了一种新的污泥衰减特性——凋亡样衰减细胞分布（ALDCD）来描述污泥衰减。污泥 F 的 ALDCD 比污泥 M 和 S 分别低 6.64%和 13.5%。微生物群落和功能预测表明，污泥 F 具有最高的有机去除微生物潜力，污泥 M 具有最高的氮代谢潜力，而污泥 S 则两者潜力均最低。我们的分析表明，通过保留紧凑的污泥絮体并去除分散的污泥絮体，可以提高处理效率。本研究还促进了从污水处理中去除衰减污泥中的功能微生物群的鉴定和消除。
