Chen Zhiqiang, Mi Xianjun, Chen Ang, Duan Lifeng, Dai Xinzhen, Deng Wentong
Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical University, Zhongshan 528400, Guangdong Province, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2018 Feb 25;47(4):362-366. doi: 10.3785/j.issn.1008-9292.2018.08.06.
To investigate paraffin section thickness in immunohistochemical detection of p16 expression in cervical tissue samples.
p16 immunohistochemical staining was performed in 150 cases of chronic cervicitis, 126 cases of low-grade squamous intraepithelial lesions(LSIL), 96 cases of high-grade squamous intraepithelial lesions (HSIL) and 78 cases of cervical cancer from January 2014 to March 2018 in Zhongshan Boai Hospital. The results of p16 protein expression in paraffin sections with thickness of 2, 3, 4, 5 and 6 μm were compared using Logistic regression analysis.
With the increase of slice thickness, the staining of p16 protein in nucleus gradually increased. The thickness of cervical slices in chronic cervicitis and cervical cancer samples had no significant effect on the positive rate of p16 protein(=7.817 and 1.332, both >0.05), while the thickness of slices in LSIL and HSIL samples had significant effect on the positive rate of p16 protein (=17.688 and 10.182, <0.05 or <0.01). The stable and reliable results were obtained when the slices were between 3 and 5 μm thick.
Paraffin sections with thickness of 3.0-5.0 μm are recommended for immnohistochemical staining of p16 protein in cervical tissue samples.
探讨宫颈组织样本免疫组化检测p16表达时石蜡切片的厚度。
对2014年1月至2018年3月在中山博爱医院就诊的150例慢性宫颈炎、126例低级别鳞状上皮内病变（LSIL）、96例高级别鳞状上皮内病变（HSIL）和78例宫颈癌患者进行p16免疫组化染色。采用Logistic回归分析比较厚度为2、3、4、5和6μm的石蜡切片中p16蛋白表达结果。
随着切片厚度增加，细胞核中p16蛋白染色逐渐增强。慢性宫颈炎和宫颈癌样本中宫颈切片厚度对p16蛋白阳性率无显著影响（χ² = 7.817和1.332，均>0.05），而LSIL和HSIL样本中切片厚度对p16蛋白阳性率有显著影响（χ² = 17.688和10.182，<0.05或<0.01）。切片厚度在3至5μm时可获得稳定可靠的结果。
宫颈组织样本p16蛋白免疫组化染色推荐使用厚度为3.0 - 5.0μm的石蜡切片。
