Graduate Program in Immunology, Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Obstetrics & Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nanomedicine. 2018 Apr;14(3):1051-1058. doi: 10.1016/j.nano.2018.01.016. Epub 2018 Feb 3.
The aim of this study was to develop a paper-based immunosensor for cervical cancer screening, with signal amplification by multifunctionalized gold nanoparticles (AuNPs). The AuNPs were functionalized with a highly specific antibody to the p16 cancer biomarker. The signal was amplified using a combination of the peroxidase activity of horseradish peroxidase (HRP) enzyme-antibody conjugate and the peroxidase-like activity of the AuNPs. The immune complex of p16 protein and multifunctionalized AuNPs was deposited on the nitrocellulose membrane, and a positive result was generated by catalytic oxidation of peroxidase enzyme substrate 3,3',5,5'-Tetramethylbenzidine (TMB). The entire reaction occurred on the membrane within 30 min. Evaluation in clinical samples revealed 85.2% accuracy with a kappa coefficient of 0.69. This proof of concept study demonstrates the successful development of a highly accurate, paper-based immunosensor that is easy to interpret using the naked eye and that is suitable for cervical cancer screening in low-resource settings.
本研究旨在开发一种基于纸张的用于宫颈癌筛查的免疫传感器，通过多功能化金纳米粒子（AuNPs）进行信号放大。AuNPs 用高度特异性的 p16 癌症生物标志物抗体进行功能化。信号通过辣根过氧化物酶（HRP）酶-抗体缀合物的过氧化物酶活性和 AuNPs 的过氧化物酶样活性的组合进行放大。p16 蛋白和多功能化 AuNPs 的免疫复合物沉积在硝酸纤维素膜上，过氧化物酶酶底物 3,3',5,5'-四甲基联苯胺（TMB）的催化氧化产生阳性结果。整个反应在 30 分钟内发生在膜上。在临床样本中的评估显示出 85.2%的准确率，kappa 系数为 0.69。这项概念验证研究表明，成功开发了一种高度准确、基于纸张的免疫传感器，使用肉眼即可轻松解读，适用于资源匮乏环境中的宫颈癌筛查。
