a Department of Sensory Science , Kawasaki University of Medical Welfare , Okayama , Japan.
b Department of Ophthalmology , Kochi Medical School , Nankoku-city , Kochi , Japan.
Curr Eye Res. 2019 Apr;44(4):376-380. doi: 10.1080/02713683.2018.1554153. Epub 2018 Dec 12.
We developed a hyperemia analysis software, which can quantitatively assess the degree of conjunctival hyperemia, and evaluated the reproducibility and reliability of its percent coverage. In addition, we compared the clinical grading and the percent coverage to examine the applicability of the software analysis.
We took images of the temporal conjunctiva with slit lamp microscopes. We used our hyperemia analysis software to detect blood vessels in the region of interest using its image processing capabilities and obtained a ratio of the area occupied by blood vessels to the rest of the area. In addition, we used the clinical evaluation criteria of the Japanese guidelines for allergic conjunctival diseases 2017 to clinically grade the hyperemia in each eye. Nine evaluators examined the images and graded the severity of hyperemia into four stages. We looked for a statistical correlation between the results of the hyperemia analysis software and the clinical grading.
The percent coverage of the blood vessels in the region of interest calculated by the hyperemia analysis software correlated with the arithmetic average of our clinical grading (r = 0.953; 95% CI, 0.8470340-0.9862136).
The percent coverage from our hyperemia analysis software reflects the clinical grading score, suggesting that our software can be used to obtain a detailed analysis of conjunctival hyperemia.
我们开发了一种充血分析软件，可以定量评估结膜充血的程度，并评估其百分比覆盖的重现性和可靠性。此外，我们比较了临床分级和百分比覆盖，以检查软件分析的适用性。
我们使用裂隙灯显微镜拍摄颞侧结膜的图像。我们使用充血分析软件的图像处理功能来检测感兴趣区域的血管，并获得血管面积与其余面积的比值。此外，我们使用 2017 年日本过敏性结膜炎指南的临床评估标准对每只眼睛的充血进行临床分级。九名评估者检查图像并将充血严重程度分为四个等级。我们寻找充血分析软件结果与临床分级之间的统计相关性。
充血分析软件计算的感兴趣区域血管的百分比覆盖与我们的临床分级的算术平均值相关（r=0.953；95%置信区间，0.8470340-0.9862136）。
我们的充血分析软件的百分比覆盖反映了临床分级评分，表明我们的软件可用于对结膜充血进行详细分析。
