Department of Chemical Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA 01854, USA.
Department of Chemical Engineering, University of Massachusetts Lowell, One University Ave, Lowell, MA 01854, USA.
Biosens Bioelectron. 2019 Feb 1;126:632-639. doi: 10.1016/j.bios.2018.10.025. Epub 2018 Oct 17.
Antibiotics such as penicillin and tetracycline drugs are widely used in food animals to treat, control, and prevent diseases, and penicillin is approved for use to improve growth rates in pigs and poultry. However, due to the overuse of antibiotics in food and medical industry, the antimicrobial resistance is starting to show up in some developing countries. The antibiotic abuse may cause allergic reactions, resistance in microorganisms and general lowering of immunity in consumers of meat and dairy products. It is important and necessary to develop an easy, inexpensive, and quantitative sensing method to monitor and analyze the antibiotics concentration in real samples such as milk or meat. In this research, an electrochemical biosensor based on hybrid nanowire/nanoparticle array with various bio-molecular receptors was fabricated for the simultaneous detection of penicillin and tetracycline. The vertically aligned Pt-Au nanowire array has been prepared by an electrodeposition method within anodic aluminum oxide (AAO) membranes; L-cysteine was used to form a monolayer on the Au segment as the bio-receptor for tetracycline detection; electroless plating of Au nanoparticles was applied on the Pt nanowire segments, and then the penicillinase was immobilized on the Au nanoparticles using EDC/NHS cross-linker. The prepared Au(L-cysteine)-Pt(penicillinase) nanowire array electrode showed simultaneous detection ability and remarkably high sensitivity of penicillin and tetracycline, which are 41.2 μA μM cm for penicillin detection and 26.4 μA μM cm for tetracycline detection. The sensitivities of each analytes with different segment length were also investigated. Real sample tests with chicken and beef extract were conducted, which showed good recovery performance. Due to the advantages of the hybrid nanowire/nanoparticle array structure, this new sensor can serve as an enhanced platform for simultaneous detection of various bioanalytes.
抗生素如青霉素和四环素类药物被广泛应用于食品动物，以治疗、控制和预防疾病，青霉素被批准用于提高猪和家禽的生长速度。然而，由于抗生素在食品和医疗行业的过度使用，抗药性开始在一些发展中国家出现。抗生素滥用可能导致消费者食用的肉类和奶制品中的过敏反应、微生物的抗药性以及总体免疫力下降。因此，开发一种简单、廉价、定量的传感方法来监测和分析牛奶或肉类等实际样品中的抗生素浓度是非常重要和必要的。在这项研究中，我们制备了一种基于混合纳米线/纳米颗粒阵列的电化学生物传感器，该传感器具有多种生物分子受体，可用于同时检测青霉素和四环素。通过在阳极氧化铝（AAO）膜内的电沉积方法制备了垂直排列的 Pt-Au 纳米线阵列；L-半胱氨酸被用于在 Au 段上形成单层作为四环素检测的生物受体；通过无电沉积法在 Pt 纳米线段上沉积 Au 纳米颗粒，然后使用 EDC/NHS 交联剂将青霉素酶固定在 Au 纳米颗粒上。所制备的 Au（L-半胱氨酸）-Pt（青霉素酶）纳米线阵列电极具有同时检测能力和对青霉素和四环素的极高灵敏度，分别为 41.2 μA μM cm 用于检测青霉素，26.4 μA μM cm 用于检测四环素。还研究了不同段长的每种分析物的灵敏度。对鸡肉和牛肉提取物进行了实际样品测试，结果显示出良好的回收率。由于混合纳米线/纳米颗粒阵列结构的优势，这种新传感器可以作为同时检测各种生物分析物的增强平台。
