Tyndall National Institute, University College Cork, Lee Maltings, Cork, Ireland.
Biosens Bioelectron. 2010 Dec 15;26(4):1420-4. doi: 10.1016/j.bios.2010.07.071. Epub 2010 Jul 27.
Novel electrochemical platform based on Pt nanoparticle modified ordered three-dimensional gold nanowire arrays (PtNP/NAEs) for the amperometric sensing of H(2)O(2) and glutamate is developed. Pt nanoparticle (PtNP) is fabricated by electrodeposition onto the 3D nanowires and characterised using scanning electron microscopy (SEM) and cyclic voltammetry. The deposited nanoparticles have an average size of 20 nm. The PtNP/NAE shows a linear response of up to 20 mM for H(2)O(2) detection with a sensitivity of 194.60 μA mM(-1) cm(-2) at 20°C. It can detect 1 μM (S/N=3) of H(2)O(2) at normal condition without using any enzyme or mediator. Analytical performance of this electrode is tested by immobilising glutamate oxidase (GlutOx) through cross-linking in the matrix of bovine serum albumin (BSA), Nafion and glutaraldehyde. At physiological pH, the biosensor showed the sensitivity of 10.76 μA mM(-1) cm(-2), with a linear range of up to 0.8 mM.
基于 Pt 纳米颗粒修饰有序三维金纳米线阵列 (PtNP/NAEs) 的新型电化学平台被开发用于安培传感 H(2)O(2) 和谷氨酸。Pt 纳米颗粒 (PtNP) 通过电沉积在 3D 纳米线上制备，并使用扫描电子显微镜 (SEM) 和循环伏安法进行表征。沉积的纳米颗粒平均尺寸为 20nm。PtNP/NAE 对 H(2)O(2)检测的线性响应高达 20mM，在 20°C 时灵敏度为 194.60μA mM(-1)cm(-2)。它可以在正常条件下无需使用任何酶或介体检测 1μM (S/N=3) 的 H(2)O(2)。通过在牛血清白蛋白 (BSA)、Nafion 和戊二醛的基质中交联固定谷氨酸氧化酶 (GlutOx)，测试了该电极的分析性能。在生理 pH 值下，生物传感器的灵敏度为 10.76μA mM(-1)cm(-2)，线性范围高达 0.8mM。
